ArticleMethods in enzymology2008
Reconstitution of RNA exosomes from human and Saccharomyces cerevisiae cloning, expression, purification, and activity assays.
Article in Methods in enzymology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 26 citations in OpenAlex.
- Substrate selectivity by the exonuclease Rrp6p.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Reconstitution of S. cerevisiae RNA Exosome Complexes Using Recombinantly Expressed Proteins.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Engineered viral RNA decay intermediates to assess XRN1-mediated decay.Methods (San Diego, Calif.) · 2019Article
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- Mpp6 Incorporation in the Nuclear Exosome Contributes to RNA Channeling through the Mtr4 Helicase.Cell reports · 2017Article
- Article
- The Rrp6 C-terminal domain binds RNA and activates the nuclear RNA exosome.Nucleic acids research · 2017Article
- MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes.Methods in enzymology · 2017Article
- Nuclear RNA Exosome at 3.1 Å Reveals Substrate Specificities, RNA Paths, and Allosteric Inhibition of Rrp44/Dis3.Molecular cell · 2016Article
- Purification and analysis of endogenous human RNA exosome complexes.RNA (New York, N.Y.) · 2016Article
- Structure of a Cytoplasmic 11-Subunit RNA Exosome Complex.Molecular cell · 2016Article
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- Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement.Acta crystallographica. Section D, Biological crystallography · 2013Article
- The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro.Nucleic acids research · 2013Article
- Article
- Exo- and endoribonucleolytic activities of yeast cytoplasmic and nuclear RNA exosomes are dependent on the noncatalytic core and central channel.Molecular cell · 2012Article
- Yeast nuclear RNA processing.World journal of biological chemistry · 2012Article
- Activities of human RRP6 and structure of the human RRP6 catalytic domain.RNA (New York, N.Y.) · 2011Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic RNA exosomes participate in 3' to 5'-processing and degradation of RNA in the nucleus and cytoplasm. RNA exosomes are multisubunit complexes composed of at least nine distinct proteins that form the exosome core. Although the eukaryotic exosome core shares structural and sequence similarity to phosphorolytic archaeal exosomes and bacterial PNPase, the eukaryotic exosome core has diverged from its archaeal and bacterial cousins and appears devoid of phosphorolytic activity. In yeast, the processive hydrolytic 3' to 5'-exoribonuclease Rrp44 associates with exosomes in the nucleus and cytoplasm. Although human Rrp44 appears homologous to yeast Rrp44, it has not yet been shown to associate with human exosomes. In the nucleus, eukaryotic exosomes interact with Rrp6, a distributive hydrolytic 3' to 5'-exoribonuclease. To facilitate analysis of eukaryotic RNA exosomes, we will describe procedures used to clone, express, purify, and reconstitute the nine-subunit human exosome and nine-, ten-, and eleven-subunit yeast exosomes. We will also discuss procedures to assess exoribonuclease activity for reconstituted exosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.