ArticleJournal of virological methods2009
Rapid and sensitive detection of 68 unique varicella zoster virus gene transcripts in five multiplex reverse transcription-polymerase chain reactions.
Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 33 citations in OpenAlex.
- Elevated serum substance P during simian varicella virus infection in rhesus macaques: implications for chronic inflammation and adverse cerebrovascular events.Journal of neurovirology · 2020Article
- Capillary electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2.Journal of virological methods · 2020Article
- Multi-parameter gene expression profiling of peripheral blood for early detection of hepatocellular carcinoma.World journal of gastroenterology · 2018Article
- Development and application of a rapid molecular method for detection of fusion genes in pediatric leukemia.International journal of clinical and experimental pathology · 2018Article
- Targeted Genome Sequencing Reveals Varicella-Zoster Virus Open Reading Frame 12 Deletion.Journal of virology · 2017Article
- Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections.Diagnostic microbiology and infectious disease · 2017Article
- Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene.Journal of virology · 2016Article
- A comparison of herpes simplex virus type 1 and varicella-zoster virus latency and reactivation.The Journal of general virology · 2015Review
- Varicella Zoster Virus in the Nervous System.F1000Research · 2015Review
- Varicella-zoster.Handbook of clinical neurology · 2014Review
- GeXPS multiplex PCR analysis of the simian varicella virus transcriptome in productively infected cells in culture and acutely infected ganglia.Journal of virological methods · 2013Article
- Varicella zoster virus (VZV)-human neuron interaction.Viruses · 2013Review
- The variegate neurological manifestations of varicella zoster virus infection.Current neurology and neuroscience reports · 2013Review
- Restricted varicella-zoster virus transcription in human trigeminal ganglia obtained soon after death.Journal of virology · 2012Article
- The development of a GeXP-based multiplex reverse transcription-PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes.BMC infectious diseases · 2012Article
- A novel multiplex polymerase chain reaction assay for profile analyses of gene expression in peripheral blood.BMC cardiovascular disorders · 2012Article
- Simultaneously typing nine serotypes of enteroviruses associated with hand, foot, and mouth disease by a GeXP analyzer-based multiplex reverse transcription-PCR assay.Journal of clinical microbiology · 2012Article
- Simian varicella virus gene expression during acute and latent infection of rhesus macaques.Journal of neurovirology · 2011Article
- Review: The neurobiology of varicella zoster virus infection.Neuropathology and applied neurobiology · 2011Review
- Synthesis and decay of varicella zoster virus transcripts.Journal of neurovirology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Varicella zoster virus (VZV) becomes latent in ganglionic neurons along the entire neuraxis. Although all predicted VZV open reading frames (ORFs) have been detected by macroarray and microarray analysis in virus-infected cells in culture where virus gene expression is abundant, array technology does not detect all VZV gene transcripts in latently infected human ganglia, where the abundance of ganglionic RNA is low and VZV gene transcription is highly variable. Using reverse transcription-polymerase chain reaction (RT-PCR) and the GenomeLab Genetic Analysis System (GeXPS), transcripts mapping to all 68 predicted unique VZV ORFs were detected in VZV-infected MeWo cells. Oligonucleotide primers contained both VZV- and cell-specific sequences linked to universal DNA sequences such that PCR amplification products were of predetermined sizes. Amplification products were resolved by capillary gel electrophoresis and detected by fluorescence spectrophotometry. Serial dilutions of total RNA extracted from VZV-infected MeWo cells were analyzed in parallel by GeXPS multiplex RT-PCR and real-time RT-PCR. GeXPS technology detected as few as 20 copies of VZV gene-specific transcripts. Only five multiplex RT-PCR assays were needed to analyze the entire VZV transcriptome. This technology will allow rapid analysis of all VZV genes transcribed during latency in human ganglia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.