ArticleWorld journal of gastroenterology2008
Disruption of colonic barrier function and induction of mediator release by strains of Campylobacter jejuni that invade epithelial cells.
Article in World journal of gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Impact of Isoquinoline Alkaloids on the Intestinal Barrier in a Colonic Model ofInternational journal of molecular sciences · 2025Article
- Campylobacter jejuni genotypes are associated with post-infection irritable bowel syndrome in humans.Communications biology · 2021Article
- Diarrheal Mechanisms and the Role of Intestinal Barrier Dysfunction in Campylobacter Infections.Current topics in microbiology and immunology · 2021Article
- Gut Microbiota Features Associated With Campylobacter Burden and Postnatal Linear Growth Deficits in a Peruvian Birth Cohort.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Article
- Cellular Tight Junctions Prevent EffectiveFrontiers in cellular and infection microbiology · 2018Article
- IntestinalFrontiers in physiology · 2018Review
- Causal Pathways from Enteropathogens to Environmental Enteropathy: Findings from the MAL-ED Birth Cohort Study.EBioMedicine · 2017Article
- Lactobacillus gasseri SBT2055 reduces infection by and colonization of Campylobacter jejuni.PloS one · 2014Article
- Transmigration route of Campylobacter jejuni across polarized intestinal epithelial cells: paracellular, transcellular or both?Cell communication and signaling : CCS · 2013Review
- The chronic gastrointestinal consequences associated with campylobacter.Current gastroenterology reports · 2012Review
- Campylobacter polysaccharide capsules: virulence and vaccines.Frontiers in cellular and infection microbiology · 2012Review
- Induction of increased permeability of polarized enterocyte monolayers by enterotoxigenic Escherichia coli heat-labile enterotoxin.The American journal of tropical medicine and hygiene · 2011Article
- Probiotic colonization of the adherent mucus layer of HT29MTXE12 cells attenuates Campylobacter jejuni virulence properties.Infection and immunity · 2010Article
- Induction of a chemoattractant transcriptional response by a Campylobacter jejuni boiled cell extract in colonocytes.BMC microbiology · 2009Article
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Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
aimTo study the mechanisms by which Campylobacter jejuni (C. jejuni) causes inflammation and diarrhea. In particular, direct interactions with intestinal epithelial cells and effects on barrier function are poorly under-stood.
methodsTo model the initial pathogenic effects of C. jejuni on intestinal epithelium, polarized human colonic HCA-7 monolayers were grown on permeabilized filters and infected apically with clinical isolates of C. jejuni. Integrity of the monolayer was monitored by changes in monolayer resistance, release of lactate dehydrogenase, mannitol fluxes and electron microscopy. Invasion of HCA-7 cells was assessed by a modified gentamicin protection assay, translocation by counting colony forming units in the basal chamber, stimulation of mediator release by immunoassays and secretory responses in monolayers stimulated by bradykinin in an Ussing chamber.
resultsAll strains translocated across monolayers but only a minority invaded HCA-7 cells. Strains that invaded HCA-7 cells destroyed monolayer resistance over 6 h, accompanied by increased release of lactate dehydrogenase, a four-fold increase in permeability to [(3)H] mannitol, and ultrastructural disruption of tight junctions, with rounding and lifting of cells off the filter membrane. Synthesis of interleukin (IL)-8 and prostaglandin E(2) was increased with strains that invaded the monolayer but not with those that did not.
conclusionThese data demonstrate two distinct effects of C. jejuni on colonic epithelial cells and provide an informative model for further investigation of initial host cell responses to C. jejuni.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.