ArticleBlood2009
Functional genomics in zebrafish permits rapid characterization of novel platelet membrane proteins.
Article in Blood, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
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- Relationship between biomarkers and subsequent bleeding risk in ST-segment elevation myocardial infarction patients treated with paclitaxel-eluting stents: a HORIZONS-AMI substudy.Journal of thrombosis and thrombolysis · 2013Trial
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- Wuliangye Baijiu but not ethanol reduces cardiovascular disease risks in a zebrafish thrombosis model.NPJ science of food · 2022Article
- A homozygous nonsense mutation in DCBLD2 is a candidate cause of developmental delay, dysmorphic features and restrictive cardiomyopathy.Scientific reports · 2021Article
- Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade.Frontiers in pharmacology · 2021Article
- A genetic modifier of venous thrombosis in zebrafish reveals a functional role for fibrinogen AαE in early hemostasis.Blood advances · 2020Article
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- Association of DCBLD2 upregulation with tumor progression and poor survival in colorectal cancer.Cellular oncology (Dordrecht, Netherlands) · 2020Article
- Zebrafish for thrombocytopoiesis- and hemostasis-related researches and disorders.Blood science (Baltimore, Md.) · 2020Review
- Defective fibrin deposition and thrombus stability in BambiJournal of thrombosis and haemostasis : JTH · 2019Article
- Intracellular and extracellular TGF-β signaling in cancer: some recent topics.Frontiers of medicine · 2018Review
- Functional Genomics for the Identification of Modulators of Platelet-Dependent Thrombus Formation.TH open : companion journal to thrombosis and haemostasis · 2018Review
- Immunoregulatory functions and the therapeutic implications of GARP-TGF-β in inflammation and cancer.Journal of hematology & oncology · 2018Review
- Blocking immunosuppression by human Tregs in vivo with antibodies targeting integrin αVβ8.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Establishment of a congenital amegakaryocytic thrombocytopenia model and a thrombocyte-specific reporter line in zebrafish.Leukemia · 2017Article
- Article
- Temporally coordinated signals progressively pattern the anteroposterior and dorsoventral body axes.Seminars in cell & developmental biology · 2015Review
- Modeling Disorders of Blood Coagulation in the Zebrafish.Current pathobiology reports · 2015Article
- Zebrafish as a model system for the study of hemostasis and thrombosis.Current opinion in hematology · 2014Review
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Authors and funding
18 authors at 10 institutions in 3 countries.
Funding
Abstract
In this study, we demonstrate the suitability of the vertebrate Danio rerio (zebrafish) for functional screening of novel platelet genes in vivo by reverse genetics. Comparative transcript analysis of platelets and their precursor cell, the megakaryocyte, together with nucleated blood cell elements, endothelial cells, and erythroblasts, identified novel platelet membrane proteins with hitherto unknown roles in thrombus formation. We determined the phenotype induced by antisense morpholino oligonucleotide (MO)-based knockdown of 5 of these genes in a laser-induced arterial thrombosis model. To validate the model, the genes for platelet glycoprotein (GP) IIb and the coagulation protein factor VIII were targeted. MO-injected fish showed normal thrombus initiation but severely impaired thrombus growth, consistent with the mouse knockout phenotypes, and concomitant knockdown of both resulted in spontaneous bleeding. Knockdown of 4 of the 5 novel platelet proteins altered arterial thrombosis, as demonstrated by modified kinetics of thrombus initiation and/or development. We identified a putative role for BAMBI and LRRC32 in promotion and DCBLD2 and ESAM in inhibition of thrombus formation. We conclude that phenotypic analysis of MO-injected zebrafish is a fast and powerful method for initial screening of novel platelet proteins for function in thrombosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.