ArticleJournal of clinical microbiology2009
Utilization of microsatellite polymorphism for differentiating herpes simplex virus type 1 strains.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
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- Comparison of microsatellite distribution in the genomes of Pteropus vampyrus and Miniopterus natalensis (Chiroptera).BMC genomic data · 2023Article
- Genome-wide identification and characterization of microsatellite markers within the Avipoxviruses.3 Biotech · 2022Article
- Genome-wideGene reports · 2021Article
- A holistic perspective on herpes simplex virus (HSV) ecology and evolution.Advances in virus research · 2021Review
- Comparison of the Microsatellite Distribution Patterns in the Genomes of Euarchontoglires at the Taxonomic Level.Frontiers in genetics · 2021Article
- Comparative analysis, distribution, and characterization of microsatellites in Orf virus genome.Scientific reports · 2020Article
- Genomic analysis ofPhysiological and molecular plant pathology · 2020Article
- Genomewide analysis of microsatellite markers based on sequenced database in two anuran species.Journal of genetics · 2020Article
- Genotyping of Salmon Gill Poxvirus Reveals One Main Predominant Lineage in Europe, Featuring Fjord- and Fish Farm-Specific Sub-Lineages.Frontiers in microbiology · 2020Article
- Microsatellite Diversity, Complexity, and Host Range of Mycobacteriophage Genomes of theFrontiers in genetics · 2019Article
- Intra-epidemic genome variation in highly pathogenic African swine fever virus (ASFV) from the country of Georgia.Virology journal · 2018Article
- Distribution patterns and variation analysis of simple sequence repeats in different genomic regions of bovid genomes.Scientific reports · 2018Article
- Genomic and biologic comparisons of cyprinid herpesvirus 3 strains.Veterinary research · 2018Article
- Inferred father-to-son transmission of herpes simplex virus results in near-perfect preservation of viral genome identity and in vivo phenotypes.Scientific reports · 2017Article
- Genome-wide In Silico Analysis, Characterization and Identification of Microsatellites in Spodoptera littoralis Multiple nucleopolyhedrovirus (SpliMNPV).Scientific reports · 2016Article
- Genomic, phylogenetic, and recombinational characterization of herpes simplex virus 2 strains.Journal of virology · 2015Article
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- In- silico exploration of thirty alphavirus genomes for analysis of the simple sequence repeats.Meta gene · 2014Article
- Molecular method development and establishment of a database for clinical and epidemiological herpes simplex virus 1 strain comparisons.Journal of clinical microbiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nucleotide motifs; they are often highly variable and polymorphic within the genome, which raises the question of whether they may be used as molecular markers for the precise differentiation of HSV-1 strains. In this study, 79 different microsatellites (mono-, di-, and trinucleotide repeats) in the HSV-1 complete genome were identified by in silico analysis. Among those microsatellites, 45 were found to be distributed in intergenic or noncoding inverted repeat regions, while 34 were in open reading frames. Length polymorphism analysis of the PCR products was used to investigate a set of 12 distinct HSV-1 strains and allowed the identification of 23 polymorphic and 6 monomorphic microsatellites, including two polymorphic trinucleotide repeats (CGT and GGA) within the UL46 and US4 genes, respectively. A multiplex PCR method that amplified 10 polymorphic microsatellites was then developed for the rapid and accurate genetic characterization of HSV-1 strains. Each HSV-1 strain was characterized by its own microsatellite haplotype, which proved to be stable over time in cell culture. This relevant innovative tool was successfully applied both to confirm the close relationship between sequential HSV-1 isolates collected from patients with multiple recurrent infections and to investigate putative nosocomial infections.
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