ArticleMolecular biology of the cell2009
SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 66 citations in OpenAlex.
- ELAPOR1 regulates VPS54-mediated GARP complex formation and proacrosomal vesicle fusion during spermatogenesis.Theranostics · 2026Article
- The primate-specific Nedd4-1(NE) localizes to late endosomes in response to amino acids to suppress autophagy.Nature communications · 2025Article
- UtilizingJournal of developmental biology · 2025Review
- Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections.G3 (Bethesda, Md.) · 2021Article
- VPS33B suppresses lung adenocarcinoma metastasis and chemoresistance to cisplatin.Genes & diseases · 2021Article
- Vps13b is required for acrosome biogenesis through functions in Golgi dynamic and membrane trafficking.Cellular and molecular life sciences : CMLS · 2020Article
- Mechanism of platelet α-granule biogenesis: study of cargo transport and the VPS33B-VPS16B complex in a model system.Blood advances · 2019Article
- Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.PLoS genetics · 2019Article
- Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles.Journal of cell science · 2018Article
- Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.Journal of molecular biology · 2018Article
- Vps3 and Vps8 control integrin trafficking from early to recycling endosomes and regulate integrin-dependent functions.Nature communications · 2018Article
- Review
- Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.Nature communications · 2016Article
- Membrane Tethering Complexes in the Endosomal System.Frontiers in cell and developmental biology · 2016Review
- Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.The Journal of biological chemistry · 2015Article
- Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.Traffic (Copenhagen, Denmark) · 2015Article
- Arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome: from molecular genetics to clinical features.Italian journal of pediatrics · 2014Review
- Atg7 is required for acrosome biogenesis during spermatogenesis in mice.Cell research · 2014Article
- The regulation of spermatogenesis and sperm function in nematodes.Seminars in cell & developmental biology · 2014Review
- Caenorhabditis elegans HOPS and CCZ-1 mediate trafficking to lysosome-related organelles independently of RAB-7 and SAND-1.Molecular biology of the cell · 2014Article
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9 authors at 1 institution in 1 country.
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Abstract
Yeast and animal homotypic fusion and vacuole protein sorting (HOPS) complexes contain conserved subunits, but HOPS-mediated traffic in animals might require additional proteins. Here, we demonstrate that SPE-39 homologues, which are found only in animals, are present in RAB5-, RAB7-, and RAB11-positive endosomes where they play a conserved role in lysosomal delivery and probably function via their interaction with the core HOPS complex. Although Caenorhabditis elegans spe-39 mutants were initially identified as having abnormal vesicular biogenesis during spermatogenesis, we show that these mutants also have disrupted processing of endocytosed proteins in oocytes and coelomocytes. C. elegans SPE-39 interacts in vitro with both VPS33A and VPS33B, whereas RNA interference of VPS33B causes spe-39-like spermatogenesis defects. The human SPE-39 orthologue C14orf133 also interacts with VPS33 homologues and both coimmunoprecipitates and cosediments with other HOPS subunits. SPE-39 knockdown in cultured human cells altered the morphology of syntaxin 7-, syntaxin 8-, and syntaxin 13-positive endosomes. These effects occurred concomitantly with delayed mannose 6-phosphate receptor-mediated cathepsin D delivery and degradation of internalized epidermal growth factor receptors. Our findings establish that SPE-39 proteins are a previously unrecognized regulator of lysosomal delivery and that C. elegans spermatogenesis is an experimental system useful for identifying conserved regulators of metazoan lysosomal biogenesis.
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