ArticleMolecular biology of the cell2009
Systematic analysis in Caenorhabditis elegans reveals that the spindle checkpoint is composed of two largely independent branches.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 93 citations in OpenAlex.
- NPP-21/TPR is required for developmental control of spindle checkpoint strength inbioRxiv : the preprint server for biology · 2026Article
- Fidelity-Ensuring Consistency of Mitosis is Safeguarded by the 53BP1-USP28-p53 Pathway.bioRxiv : the preprint server for biology · 2026Article
- A simple fixation for detection of endogenous fluorescent reporters inmicroPublication biology · 2025Article
- Low-fecunditymicroPublication biology · 2025Article
- Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses.The Journal of cell biology · 2024Article
- Kinetochore dynein is sufficient to biorient chromosomes and remodel the outer kinetochore.Nature communications · 2024Article
- Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.Journal of cell science · 2024Article
- A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans.Nature communications · 2024Article
- Phospho-KNL-1 recognition by a TPR domain targets the BUB-1-BUB-3 complex to C. elegans kinetochores.The Journal of cell biology · 2024Article
- The matrisome landscape controlling in vivo germ cell fates.Nature communications · 2024Article
- Chromosome Division in Early Embryos-Is Everything under Control? And Is the Cell Size Important?International journal of molecular sciences · 2024Review
- Loss of the puromycin-sensitive aminopeptidase, PAM-1, triggers the spindle assembly checkpoint during the first mitotic division inmicroPublication biology · 2024Article
- Kinetochore component function in C. elegans oocytes revealed by 4D tracking of holocentric chromosomes.Nature communications · 2023Article
- BUB-1-bound PLK-1 directs CDC-20 kinetochore recruitment to ensure timely embryonic mitoses.Current biology : CB · 2023Article
- Article
- Mad1's ability to interact with Mad2 is essential to regulate and monitor meiotic synapsis in C. elegans.PLoS genetics · 2021Article
- Spindle assembly checkpoint activation and silencing at kinetochores.Seminars in cell & developmental biology · 2021Review
- Bub1 kinase in the regulation of mitosis.Animal cells and systems · 2021Review
- A tripartite mechanism catalyzes Mad2-Cdc20 assembly at unattached kinetochores.Science (New York, N.Y.) · 2021Article
- Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Kinetochores use the spindle checkpoint to delay anaphase onset until all chromosomes have formed bipolar attachments to spindle microtubules. Here, we use controlled monopolar spindle formation to systematically define the requirements for spindle checkpoint signaling in the Caenorhabditis elegans embryo. The results, when interpreted in light of kinetochore assembly epistasis analysis, indicate that checkpoint activation is coordinately directed by the NDC-80 complex, the Rod/Zwilch/Zw10 complex, and BUB-1-three components independently targeted to the outer kinetochore by the scaffold protein KNL-1. These components orchestrate the integration of a core Mad1(MDF-1)/Mad2(MDF-2)-based signal, with a largely independent Mad3(SAN-1)/BUB-3 pathway. Evidence for independence comes from the fact that subtly elevating Mad2(MDF-2) levels bypasses the requirement for BUB-3 and Mad3(SAN-1) in kinetochore-dependent checkpoint activation. Mad3(SAN-1) does not accumulate at unattached kinetochores and BUB-3 kinetochore localization is independent of Mad2(MDF-2). We discuss the rationale for a bipartite checkpoint mechanism in which a core Mad1(MDF-1)/Mad2(MDF-2) signal generated at kinetochores is integrated with a separate cytoplasmic Mad3(SAN-1)/BUB-3-based pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.