ArticleJournal of immunology (Baltimore, Md. : 1950)2009
E2A acts in cis in G1 phase of cell cycle to promote Ig gene diversification.
Article in Journal of immunology (Baltimore, Md. : 1950), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Helix-Loop-Helix Proteins in Adaptive Immune Development.Frontiers in immunology · 2022Review
- Ig Enhancers Increase RNA Polymerase II Stalling at Somatic Hypermutation Target Sequences.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Immunoglobulin Expression in Cancer Cells and Its Critical Roles in Tumorigenesis.Frontiers in immunology · 2021Review
- Transcription factor binding at Ig enhancers is linked to somatic hypermutation targeting.European journal of immunology · 2020Article
- Interstitial telomeric repeats-associated DNA breaks.Nucleus (Austin, Tex.) · 2017Article
- Cell Cycle Regulates Nuclear Stability of AID and Determines the Cellular Response to AID.PLoS genetics · 2015Article
- A critical context-dependent role for E boxes in the targeting of somatic hypermutation.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Identification of core DNA elements that target somatic hypermutation.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Article
- Separation of mutational and transcriptional enhancers in Ig genes.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Targeting of AID-mediated sequence diversification to immunoglobulin genes.Current opinion in immunology · 2011Review
- Article
- Distinct regulatory mechanism of immunoglobulin gene transcription in epithelial cancer cells.Cellular & molecular immunology · 2010Article
- RAD51 paralogs promote homology-directed repair at diversifying immunoglobulin V regions.BMC molecular biology · 2009Article
- Temporal regulation of Ig gene diversification revealed by single-cell imaging.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Targeted gene therapies: tools, applications, optimization.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Rearranged Ig genes undergo diversification in sequence and structure initiated by the DNA deaminase, activation-induced deaminase. Ig genes must be transcribed for diversification to occur, but whether there are additional requirements for cis activation has not been established. Here we show, by chromatin immunoprecipitation, that the regulatory factor E2A associates with the rearranged Ig lambda(R) gene in the chicken DT40 B cell line, which performs constitutive Ig gene diversification. By analysis of a DT40 derivative in which polymerized lactose operator tags the rearranged lambda(R) gene, we show that E2A must function in cis to promote diversification and that stimulation of diversification in cis depends on the E2A activation domains. By direct imaging, we show that lambda(R)/E2A colocalizations are most prominent in G(1). We further show that expression of the E2A antagonist Id1 prevents lambda(R)/E2A colocalizations in G(1) and impairs diversification but not transcription of lambda(R). Thus, E2A acts in cis to promote Ig gene diversification, and G(1) phase is the critical window for E2A action.
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