ArticleMolecular biotechnology2009
Gene cloning, expression, and characterization of the Geobacillus Thermoleovorans CCR11 thermoalkaliphilic lipase.
Article in Molecular biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Production and Characterization of Cross-Linked Aggregates ofMolecules (Basel, Switzerland) · 2021Article
- Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system.BMC biotechnology · 2020Article
- Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity.PloS one · 2020Article
- Point mutation Arg153-His at surface of Bacillus lipase contributing towards increased thermostability and ester synthesis: insight into molecular network.Molecular and cellular biochemistry · 2018Article
- Article
- Bacillus sp. JR3 esterase LipJ: A new mesophilic enzyme showing traces of a thermophilic past.PloS one · 2017Article
- Gene Cloning and Characterization of the Geobacillus thermoleovorans CCR11 Carboxylesterase CaesCCR11, a New Member of Family XV.Molecular biotechnology · 2016Article
- Molecular cloning and characterization of a thermostable lipase from deep-sea thermophile Geobacillus sp. EPT9.World journal of microbiology & biotechnology · 2015Article
- Unraveling the lipolytic activity of thermophilic bacteria isolated from a volcanic environment.BioMed research international · 2013Article
- Characterization and evolution of a metagenome-derived lipase towards enhanced enzyme activity and thermostability.Molecular and cellular biochemistry · 2013Article
- Characterization of a thermostable lipase showing loss of secondary structure at ambient temperature.Molecular biology reports · 2012Article
- Biochemical and structural characterization of two site-directed mutants of Staphylococcus xylosus lipase.Molecular biotechnology · 2010Article
- Cloning, expression, purification, and characterization of a novel esterase from Lactobacillus plantarum.Molecular biotechnology · 2010Article
- Heterologous Expression and Purification of a Heat-Tolerant Staphylococcus xylosus Lipase.Molecular biotechnology · 2010Article
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Authors and funding
5 authors.
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Abstract
The gene for a Geobacillus thermoleovorans CCR11 thermostable lipase was recovered by PCR and cloned. Four genetic constructions were designed and successfully expressed in E. coli: (i) the lipase structural gene (lipCCR11) in the PinPoint Xa vector; (ii) the lipase structural gene (lipACCR11) in the pET-28a(+) vector; (iii) the lipase structural gene minus the signal peptide (lipMatCCR11) in the pET-3b vector; and (iv) the lipase structural gene plus its own promoter (lipProCCR11) in the pGEM-T cloning vector. The lipase gene sequence analysis showed an open reading frame of 1,212 nucleotides coding for a mature lipase of 382 residues (40 kDa) plus a 22 residues signal peptide. Expression under T7 and T7lac promoter resulted in a 40- and 36-fold increase in lipolytic activity with respect to the original strain lipase. All recombinant lipases showed an optimal activity at pH 9.0, but variations were found in the temperature for maximum activity and the substrate specificity among them and when compared with the parental strain lipase, especially in the recombinant lipases that contained fusion tags. Therefore, it is important to find the appropriate expression system able to attain a high concentration of the recombinant lipase without compromising the proper folding of the protein.
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