ArticleMolecular biotechnology2009
Full-Length gene enrichment by using an optimized RNA isolation protocol in Bixa orellana recalcitrant tissues.
Article in Molecular biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Gene structure and potential regulation of the lycopene cyclase genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Gene expression profile during seed development ofFrontiers in plant science · 2023Article
- Gene expression analysis during the fruit development in dehiscent and indehiscentPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- IdentifyingFrontiers in plant science · 2022Article
- Article
- The biotechnology (genetic transformation and molecular biology) of Bixa orellana L. (achiote).Planta · 2018Review
- De novo transcriptome sequencing in Bixa orellana to identify genes involved in methylerythritol phosphate, carotenoid and bixin biosynthesis.BMC genomics · 2015Article
- Identification and expression pattern of a new carotenoid cleavage dioxygenase gene member from Bixa orellana.Journal of experimental botany · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A reliable protocol is described for isolation of large full-length cDNA from Bixa orellana mature tissues containing large quantities of pigments, phenols, and polysaccharides. This protocol involves the optimization of a commercial RNA extraction protocol in combination with a long distance reverse transcript PCR protocol. The principal advantages of this protocol are its high RNA yield and quality. The resulting RNA is suitable for RNA expression evaluation and production of large, full-length cDNA. This is the first time RNA has been isolated from all mature tissues in the tropical perennial plant B. orellana and has been proved viable for downstream applications, especially important for molecular biology studies on this economically important pigment-producing plant.
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Registered trials
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