ArticleThe Plant cell2008
Lotus japonicus CASTOR and POLLUX are ion channels essential for perinuclear calcium spiking in legume root endosymbiosis.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed.
- Nonnodulating Fagales retain the functional NODULE INCEPTION gene.The New phytologist · 2026Article
- Integrating single-cell omic techniques to resolve the spatio-temporal complexity of arbuscular mycorrhizal symbiosis.Journal of experimental botany · 2026Review
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- Paralogous LRR receptor kinases confer symbiosis specificity between arbuscular mycorrhizal and root nodule symbioses in Lotus japonicus.The New phytologist · 2026Article
- Circular RNAs in Lotus japonicus Responses to Nutrient Supply and Mesorhizobium Symbiosis.Plant, cell & environment · 2026Article
- Putting channels in their place: nucleoporins and symbiotic signaling in legumes.Plant physiology · 2026Article
- Structure-informed engineering of plant-microbe interactions.The Plant journal : for cell and molecular biology · 2026Review
- Transcriptomic Analysis Reveals the Role ofPlants (Basel, Switzerland) · 2026Article
- Autoactive MtDMI1 Reprogrammes Immunity and Development in Tomato via Ethylene Signalling.Plant biotechnology journal · 2026Article
- Plant cell surface receptors.The Plant journal : for cell and molecular biology · 2026Review
- A novel cis-element enabled bacterial uptake by plant cells.Nature plants · 2026Article
- De novo transcriptome assembly and gene expression analysis of Cnidium officinale under high-temperature conditions.BMC genomics · 2025Article
- The BRUTUS iron sensor and E3 ligase facilitates soybean root nodulation by monoubiquitination of NSP1.Nature plants · 2025Article
- Investigating biological nitrogen fixation via single-cell transcriptomics.Journal of experimental botany · 2025Review
- Ca2+ signatures in symbiosis: another level of dynamism for this key messenger.Journal of experimental botany · 2024Article
- Mycorrhizal status and host genotype interact to shape plant nutrition in field grown maize (Zea mays ssp. mays).Mycorrhiza · 2023Article
- Dancing to a different tune, can we switch from chemical to biological nitrogen fixation for sustainable food security?PLoS biology · 2023Article
- Review
- NIN-at the heart of NItrogen-fixing Nodule symbiosis.Frontiers in plant science · 2023Review
- Constitutive activation of a nuclear-localized calcium channel complex inProceedings of the National Academy of Sciences of the United States of America · 2022Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism underlying perinuclear calcium spiking induced during legume root endosymbioses is largely unknown. Lotus japonicus symbiosis-defective castor and pollux mutants are impaired in perinuclear calcium spiking. Homology modeling suggested that the related proteins CASTOR and POLLUX might be ion channels. Here, we show that CASTOR and POLLUX form two independent homocomplexes in planta. CASTOR reconstituted in planar lipid bilayers exhibited ion channel activity, and the channel characteristics were altered in a symbiosis-defective mutant carrying an amino acid replacement close to the selectivity filter. Permeability ratio determination and competition experiments reveled a weak preference of CASTOR for cations such as potassium over anions. POLLUX has an identical selectivity filter region and complemented a potassium transport-deficient yeast mutant, suggesting that POLLUX is also a potassium-permeable channel. Immunogold labeling localized the endogenous CASTOR protein to the nuclear envelope of Lotus root cells. Our data are consistent with a role of CASTOR and POLLUX in modulating the nuclear envelope membrane potential. They could either trigger the opening of calcium release channels or compensate the charge release during the calcium efflux as counter ion channels.
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