ArticleNucleic acids research2009
Sensitive isothermal detection of nucleic-acid sequence by primer generation-rolling circle amplification.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 216 citations in OpenAlex.
- Coupling Exponential Rolling Circle Amplification to Lateral Flow Devices for Point-of-Care Detection of Viral RNA.Angewandte Chemie (International ed. in English) · 2026Article
- Moderate-temperature DNA cleavage activity of TtAgo activated by dCTP/TthSSB for one-step isothermal microRNAs detection.Nature communications · 2026Article
- Transforming RNA Degradation into Accurate RNA Detection: RNase I-Assisted Rolling Circle Amplification for Clinical Samples.Angewandte Chemie (International ed. in English) · 2026Article
- Ultrasensitive non-enzymatic protein detection using proximity immunoassay with photonic resonator absorption microscopy.Npj biosensing · 2026Article
- High-Fidelity, One-Pot Nucleic Acid Amplification via OMEGA IsrB Nickase Cycling for Clinical Pathogen Detection.JACS Au · 2025Article
- One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity.Biosensors · 2025Article
- Design and Optimization of Isothermal Gene Amplification for Generation of High-Gain Oligonucleotide Products by MicroRNAs.ACS measurement science au · 2024Article
- A sensitive one-pot ROA assay for rapid miRNA detection.aBIOTECH · 2024Article
- A Novel Multiplex LAMP Assay for the Detection of Respiratory Human Adenoviruses.International journal of molecular sciences · 2024Article
- Recent advances in chemical biology tools for protein and RNA profiling of extracellular vesicles.RSC chemical biology · 2024Review
- An ultra-sensitive suboptimal protospacer adjacent motif enhanced rolling circle amplification assay based on CRISPR/Cas12a for detection of miR-183.Frontiers in bioengineering and biotechnology · 2024Article
- Review
- Ultrasensitive Detection of MicroRNA in Human Saliva via Rolling Circle Amplification Using a DNA-Decorated Graphene Oxide Sensor.ACS omega · 2023Article
- Article
- Programmable mismatch-fueled high-efficiency DNA signal amplifier.Chemical science · 2022Article
- Isothermal Amplification Technology for Disease Diagnosis.Biosensors · 2022Review
- Rapid quantification of microRNA-375 through one-pot primer-generating rolling circle amplification.The Analyst · 2022Article
- Detection of SARS-CoV-2 RNA through tandem isothermal gene amplification without reverse transcription.Analytica chimica acta · 2022Article
- CRISPR-dCas9 based DNA detection scheme for diagnostics in resource-limited settings.Nanoscale · 2022Article
- Programmable High-Speed and Hyper-Efficiency DNA Signal Magnifier.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple isothermal nucleic-acid amplification reaction, primer generation-rolling circle amplification (PG-RCA), was developed to detect specific nucleic-acid sequences of sample DNA. This amplification method is achievable at a constant temperature (e.g. 60 degrees C) simply by mixing circular single-stranded DNA probe, DNA polymerase and nicking enzyme. Unlike conventional nucleic-acid amplification reactions such as polymerase chain reaction (PCR), this reaction does not require exogenous primers, which often cause primer dimerization or non-specific amplification. Instead, 'primers' are generated and accumulated during the reaction. The circular probe carries only two sequences: (i) a hybridization sequence to the sample DNA and (ii) a recognition sequence of the nicking enzyme. In PG-RCA, the circular probe first hybridizes with the sample DNA, and then a cascade reaction of linear rolling circle amplification and nicking reactions takes place. In contrast with conventional linear rolling circle amplification, the signal amplification is in an exponential mode since many copies of 'primers' are successively produced by multiple nicking reactions. Under the optimized condition, we obtained a remarkable sensitivity of 84.5 ymol (50.7 molecules) of synthetic sample DNA and 0.163 pg (approximately 60 molecules) of genomic DNA from Listeria monocytogenes, indicating strong applicability of PG-RCA to various molecular diagnostic assays.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.