ArticleNucleic acids research2009
The A-rich RNA sequences of HIV-1 pol are important for the synthesis of viral cDNA.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
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- A de novo approach to inferring within-host fitness effects during untreated HIV-1 infection.PLoS pathogens · 2020Article
- Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication.The Journal of biological chemistry · 2019Review
- Global synonymous mutagenesis identifies cis-acting RNA elements that regulate HIV-1 splicing and replication.PLoS pathogens · 2018Article
- RNA Structure-A Neglected Puppet Master for the Evolution of Virus and Host Immunity.Frontiers in immunology · 2018Review
- Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication.Retrovirology · 2017Article
- HIV-1 tolerates changes in A-count in a small segment of the pol gene.Retrovirology · 2017Article
- The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV.PLoS pathogens · 2017Article
- Sequence-Specific Sensing of Nucleic Acids.Trends in immunology · 2017Review
- Structure-Based Alignment and Consensus Secondary Structures for Three HIV-Related RNA Genomes.PLoS computational biology · 2015Article
- Large-scale nucleotide optimization of simian immunodeficiency virus reduces its capacity to stimulate type I interferon in vitro.Journal of virology · 2014Article
- Quantifying selection against synonymous mutations in HIV-1 env evolution.Journal of virology · 2013Article
- Changes in codon-pair bias of human immunodeficiency virus type 1 have profound effects on virus replication in cell culture.Retrovirology · 2013Article
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- The biased nucleotide composition of the HIV genome: a constant factor in a highly variable virus.Retrovirology · 2012Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
The bias of A-rich codons in HIV-1 pol is thought to be a record of hypermutations in viral genomes that lack biological functions. Bioinformatic analysis predicted that A-rich sequences are generally associated with minimal local RNA structures. Using codon modifications to reduce the amount of A-rich sequences within HIV-1 genomes, we have reduced the flexibility of RNA sequences in pol to analyze the functional significance of these A-rich 'structurally poor' RNA elements in HIV-1 pol. Our data showed that codon modification of HIV-1 sequences led to a suppression of virus infectivity by 5-100-fold, and this defect does not correlate with, viral entry, viral protein expression levels, viral protein profiles or virion packaging of genomic RNA. Codon modification of HIV-1 pol correlated with an enhanced dimer stability of the viral RNA genome, which was associated with a reduction of viral cDNA synthesis both during HIV-1 infection and in a cell free reverse transcription assay. Our data provided direct evidence that the HIV-1 A-rich pol sequence is not merely an evolutionary artifact of enzyme-induced hypermutations, and that HIV-1 has adapted to rely on A-rich RNA sequences to support the synthesis of viral cDNA during reverse transcription, highlighting the utility of using 'structurally poor' RNA domains in regulating biological process.
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