ArticleThe Journal of biological chemistry2009
Context-dependent function of regulatory elements and a switch in chromatin occupancy between GATA3 and GATA2 regulate Gata2 transcription during trophoblast differentiation.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
- Arginine methyltransferase PRMT1 equipoises trophoblast development to prevent early pregnancy loss.Nature communications · 2026Article
- Signal Amplification in the HPT Axis-Evidence for Its Existence, Location, Significance, and Molecular Mechanisms.Acta physiologica (Oxford, England) · 2026Review
- GATA2 Mediates Macrophage Proliferation During Atherosclerosis.Journal of the American Heart Association · 2025Article
- A Single Trophoblast Layer Acts as the Gatekeeper at the Endothelial-Hematopoietic Crossroad in the Placenta.bioRxiv : the preprint server for biology · 2024Article
- The GATA transcriptional program dictates cell fate equilibrium to establish the maternal-fetal exchange interface and fetal development.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Novel GATA2 Protein Reporter Mouse Reveals Hematopoietic Progenitor Cell Types.Stem cell reports · 2020Article
- Placenta-Specific Genes, Their Regulation During Villous Trophoblast Differentiation and Dysregulation in Preterm Preeclampsia.International journal of molecular sciences · 2020Article
- Article
- Regulation of human trophoblast syncytialization by histone demethylase LSD1.The Journal of biological chemistry · 2019Article
- Regulation of energy metabolism during early mammalian development: TEAD4 controls mitochondrial transcription.Development (Cambridge, England) · 2018Article
- Review
- GATA2/3-TFAP2A/C transcription factor network couples human pluripotent stem cell differentiation to trophectoderm with repression of pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transcriptional control ofReproduction (Cambridge, England) · 2017Review
- Proximal GATA-binding sites are essential for human HSD3B1 gene transcription in the placenta.Scientific reports · 2017Article
- Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development.Development (Cambridge, England) · 2017Article
- Identification of a novel distal regulatory element of the human Neuroglobin gene by the chromosome conformation capture approach.Nucleic acids research · 2017Article
- Intrauterine trophoblast migration: A comparative view of humans and rodents.Cell adhesion & migration · 2016Review
- GATA3 inhibits GCM1 activity and trophoblast cell invasion.Scientific reports · 2016Article
- Making the first decision: lessons from the mouse.Reproductive medicine and biology · 2015Review
- Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1.The Journal of biological chemistry · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
GATA transcription factors are important regulators of tissue-specific gene expression during development. GATA2 and GATA3 have been implicated in the regulation of trophoblast-specific genes. However, the regulatory mechanisms of GATA2 expression in trophoblast cells are poorly understood. In this study, we demonstrate that Gata2 is transcriptionally induced during trophoblast giant cell-specific differentiation. Transcriptional induction is associated with displacement of GATA3-dependent nucleoprotein complexes by GATA2-dependent nucleoprotein complexes at two regulatory regions, the -3.9- and +9.5-kb regions, of the mouse Gata2 locus. Analyses with reporter genes showed that, in trophoblast cells, -3.9- and +9.5-kb regions function as transcriptional enhancers in GATA motif independent and dependent fashions, respectively. We also found that knockdown of GATA3 by RNA interference induces GATA2 in undifferentiated trophoblast cells. Interestingly, three other known GATA motif-dependent Gata2 regulatory elements, the -1.8-, -2.8-, and -77-kb regions, which are important to regulate Gata2 in hematopoietic cells are not occupied by GATA factors in trophoblast cells. These elements do not show any enhancer activity and also possess inaccessible chromatin structure in trophoblast cells indicating a context-dependent function. Our results indicate that GATA3 directly represses Gata2 in undifferentiated trophoblast cells, and a switch in chromatin occupancy between GATA3 and GATA2 (GATA3/GATA2 switch) induces transcription during trophoblast differentiation. We predict that this GATA3/GATA2 switch is an important mechanism for the transcriptional regulation of other trophoblast-specific genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.