ArticleBreast cancer research and treatment2009
Immunohistochemical validation of multiple phospho-specific epitopes for estrogen receptor alpha (ERalpha) in tissue microarrays of ERalpha positive human breast carcinomas.
Article in Breast cancer research and treatment, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Oestrogen receptor phosphorylation profiles and in silico PAM50 subtyping reflect sexual dimorphism in breast cancer.The journal of pathology. Clinical research · 2026Article
- Overlap of Formalin-Fixed Paraffin-Embedded and Fresh-Frozen Matched Tissues for Proteomics and Phosphoproteomics.ACS omega · 2025Article
- Autophagy Analysis: A Step-by-Step Simple Practical Guide for Immunofluorescence and Western Blotting.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Guidelines on antibody use in physiology research.American journal of physiology. Renal physiology · 2024Article
- Immunofluorescence Staining of Paraffin Sections Step by Step.Frontiers in neuroanatomy · 2020Article
- Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells.The Journal of cell biology · 2018Article
- Androgen Receptor and Ki67 Expression and Survival Outcomes in Non-small Cell Lung Cancer.Hormones & cancer · 2018Article
- Lipopolysaccharide (LPS) Accumulates in Neocortical Neurons of Alzheimer's Disease (AD) Brain and Impairs Transcription in Human Neuronal-Glial Primary Co-cultures.Frontiers in aging neuroscience · 2017Article
- Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips.Journal of pathology and translational medicine · 2016Review
- Phosphorylation of Farnesoid X Receptor at Serine 154 Links Ligand Activation With Degradation.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Facilitating the Validation of Novel Protein Biomarkers for Dementia: An Optimal Workflow for the Development of Sandwich Immunoassays.Frontiers in neurology · 2015Review
- Mouse ENU Mutagenesis to Understand Immunity to Infection: Methods, Selected Examples, and Perspectives.Genes · 2014Review
- The mechanistic target for rapamycin pathway is related to the phosphorylation score for estrogen receptor-α in human breast tumors in vivo.Breast cancer research : BCR · 2014Article
- Estrogen increases secretion of stromal cell derived factor-1 in human breast cancer cells.International journal of clinical and experimental medicine · 2014Article
- PKA phosphorylation redirects ERα to promoters of a unique gene set to induce tamoxifen resistance.Oncogene · 2013Article
- Ligand binding promotes CDK-dependent phosphorylation of ER-alpha on hinge serine 294 but inhibits ligand-independent phosphorylation of serine 305.Molecular cancer research : MCR · 2012Article
- Phosphorylation of human estrogen receptor-beta at serine 105 inhibits breast cancer cell migration and invasion.Molecular and cellular endocrinology · 2012Article
- Estrogen receptor-beta and breast cancer: translating biology into clinical practice.Steroids · 2012Review
- Post-translational modifications of nuclear receptors and human disease.Nuclear receptor signaling · 2012Review
- The different roles of ER subtypes in cancer biology and therapy.Nature reviews. Cancer · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Estrogen receptor alpha (ERalpha) activity is regulated by phosphorylation at several sites. Recently several antibodies specific for individual phosphorylated sites within ERalpha have became available. Such antibodies potentially provide invaluable tools to gain insight into the relevance in vivo of phosphorylated ERalpha in human breast tumors. However, validation of these antibodies for immunohistochemistry in particular is necessary in the first instance. In this study we have investigated the usefulness of several antibodies generated to specific phosphorylated sites within ERalpha for immunohistochemistry of formalin-fixed, paraffin-embedded human breast cancer biopsy samples. As well, these data demonstrate for the first time, the detection of multiple phosphorylated ERalpha forms in breast cancer (P-S104/106-ERalpha, P-S118-ERalpha, P-S167-ERalpha, P-S282-ERalpha, P-S294-ERalpha, P-T311-ERalpha, and P-S559-ERalpha) suggesting the possibility that profiling of phosphorylated ERalpha isoforms might be useful in selecting subgroups of breast cancer patients that would benefit from endocrine therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.