ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2009
Identification of fungal species by fragment length analysis of the internally transcribed spacer 2 region.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Prevalence of fungal DNAemia mediated by putatively non-pathogenic fungi in immunocompromised patients with febrile neutropenia: a prospective cohort study.Journal of hematology & oncology · 2024Article
- Oral microbial diversity analysis among atrophic glossitis patients and healthy individuals.Journal of oral microbiology · 2021Article
- Mycobiome in the Gut: A Multiperspective Review.Mediators of inflammation · 2020Review
- Candida parapsilosis and Candida tropicalis infections in an Okhotsk snailfish (Liparis ochotensis).The Journal of veterinary medical science · 2018Article
- Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections.Journal of clinical microbiology · 2016Article
- Oral mycobiome analysis of HIV-infected patients: identification of Pichia as an antagonist of opportunistic fungi.PLoS pathogens · 2014Article
- Outbreak of fungal endophthalmitis due to Fusarium oxysporum following cataract surgery.Mycopathologia · 2014Article
- A quick and low-cost PCR-based assay for Candida spp. identification in positive blood culture bottles.BMC infectious diseases · 2013Article
- CloVR-ITS: Automated internal transcribed spacer amplicon sequence analysis pipeline for the characterization of fungal microbiota.Microbiome · 2013Article
- Laboratory diagnostics of invasive fungal infections: an overview with emphasis on molecular approach.Folia microbiologica · 2012Review
- Biopsy procedures for molecular tissue diagnosis of invasive fungal infections.Current infectious disease reports · 2011Article
- Δ-PCR, A Simple Method to Detect Translocations and Insertion/Deletion Mutations.The Journal of molecular diagnostics : JMD · 2011Article
- Characterization of the oral fungal microbiome (mycobiome) in healthy individuals.PLoS pathogens · 2010Article
- Diagnosis ofCurrent fungal infection reportsReview
- Review
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Authors and funding
6 authors.
Funding
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Abstract
The rapid identification of fungal pathogens in clinical specimens is a prerequisite for timely onset of the most appropriate treatment. The aim of the present study was to develop a sensitive and rapid method for the species-specific identification of clinically relevant fungi. We employed fluorescent polymerase chain reaction (PCR)-fragment length analysis of the highly variable internally transcribed spacer 2 (ITS2) region to identify individual fungal species by their specific amplicon sizes. The specificity of the technique was ascertained by the detailed analysis of 96 strains derived from 60 different human-pathogenic fungal species. To achieve adequate sensitivity for species identification in patients with invasive fungal infection, who often display very low pathogen loads in peripheral blood, the ITS2 region was amplified by semi-nested PCR prior to amplicon-length analysis. Serial specimens from 26 patients with documented fungal infections were investigated. The fungal pathogens identified included different Aspergillus and Candida species, Rhizopus oryzae and Fusarium oxysporum. Fragment length analysis of the ITS2 region upon amplification by semi-nested PCR permits the sensitive identification of fungal species. The technique can be readily implemented in routine diagnostics.
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