ArticleInfection and immunity2009
GbdR regulates Pseudomonas aeruginosa plcH and pchP transcription in response to choline catabolites.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 69 citations in OpenAlex.
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- PvrA-Mediated Inhibition of Choline and Ethanolamine Uptake PromotesPathogens (Basel, Switzerland) · 2026Article
- Structural insights into a conserved mechanism of choline translocation through CHT.Science advances · 2026Article
- A choline-sensing regulator coordinates metabolic adaptation and pathogenesis inScience advances · 2025Article
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- Dual roles of glycine betaine, dimethylglycine, and sarcosine as osmoprotectants and nutrient sources forApplied and environmental microbiology · 2025Article
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- Metabolic and genotypic characterization of meropenem-susceptible and meropenem-resistantTurkish journal of medical sciences · 2025Article
- Choline metabolism modulates cyclic-di-GMP signaling and virulence of Pseudomonas aeruginosa in a macrophage infection model.BMC infectious diseases · 2024Article
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- Sphingosine induction of theJournal of bacteriology · 2024Article
- Phospholipase C: underrated players in microbial infections.Frontiers in cellular and infection microbiology · 2023Review
- Creatine utilization as a sole nitrogen source inMicrobiology (Reading, England) · 2022Article
- An updated gene regulatory network reconstruction of multidrug-resistant Pseudomonas aeruginosa CCBH4851.Memorias do Instituto Oswaldo Cruz · 2022Article
- Virulence factors in multidrug (MDR) and Pan-drug resistant (XDR) Pseudomonas aeruginosa: a cross-sectional study of isolates recovered from ocular infections in a high-incidence setting in southern India.Journal of ophthalmic inflammation and infection · 2021Article
- PvrA is a novel regulator that contributes to Pseudomonas aeruginosa pathogenesis by controlling bacterial utilization of long chain fatty acids.Nucleic acids research · 2020Article
- Transcriptional profiling of Vibrio cholerae O1 following exposure to human anti- lipopolysaccharide monoclonal antibodies.Pathogens and disease · 2020Article
- Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Pseudomonas aeruginosa hemolytic phospholipase C, PlcH, can degrade phosphatidylcholine (PC) and sphingomyelin in eukaryotic cell membranes and extracellular PC in lung surfactant. Numerous studies implicate PlcH in P. aeruginosa virulence. The phosphorylcholine released by PlcH activity on phospholipids is hydrolyzed by a periplasmic phosphorylcholine phosphatase, PchP. Both plcH gene expression and PchP enzyme activity are positively regulated by phosphorylcholine degradation products, including glycine betaine. Here we report that the induction of plcH and pchP transcription by glycine betaine is mediated by GbdR, an AraC family transcription factor. Mutants that lack gbdR are unable to induce plcH and pchP in media containing glycine betaine or choline and in phosphatidylcholine-rich environments, such as lung surfactant or mouse lung lavage fluid. In T broth containing choline, the gbdR mutant exhibited a 95% reduction in PlcH activity. In electrophoretic mobility shift assays, a GbdR-maltose binding protein fusion bound specifically to both the plcH and pchP promoters. Promoter mapping, alignment of GbdR-regulated promoter sequences, and analysis of targeted promoter mutants that lack GbdR-dependent induction of transcription were used to identify a region necessary for GbdR-dependent transcriptional activation. GbdR also plays a significant role in plcH and pchP regulation within the mouse lung. Our studies suggest that GbdR is the primary regulator of plcH and pchP expression in PC-rich environments, such as the lung, and that pchP and other genes involved in phosphorylcholine catabolism are necessary to stimulate the GbdR-mediated positive feedback induction of plcH.
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