ArticleMolecular and cellular biology2009
Differential contribution of the Gata1 gene hematopoietic enhancer to erythroid differentiation.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 49 citations in OpenAlex.
- Epigenetic programming by H3K23ac defines lineage fate of Meg3Nature cell biology · 2026Article
- Resolution of inflammation increases erythropoiesis.Blood red cells & iron · 2025Article
- GATA Transcription Factors: A Cross-Road for Erythropoiesis, Neurodevelopment, and Synucleinopathies.Developmental neurobiology · 2025Review
- Murine erythroid differentiation kinetics in vivo under normal and anemic stress conditions.Blood advances · 2023Article
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- Lipopolysaccharide-induced expansion of histidine decarboxylase-expressing Ly6GScientific reports · 2019Article
- DivergentBiology open · 2018Article
- Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation.The Journal of experimental medicine · 2018Article
- Inhibition of MEK/ERK signalling pathway promotes erythroid differentiation and reduces HSCs engraftment in ex vivo expanded haematopoietic stem cells.Journal of cellular and molecular medicine · 2018Article
- Mechanisms of erythrocyte development and regeneration: implications for regenerative medicine and beyond.Development (Cambridge, England) · 2018Review
- GATA2 regulates the erythropoietin receptor in t(12;21) ALL.Oncotarget · 2017Article
- Derepression of the DNA Methylation Machinery of theMolecular and cellular biology · 2017Article
- Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches.Journal of visualized experiments : JoVE · 2017Article
- Systematic mapping of functional enhancer-promoter connections with CRISPR interference.Science (New York, N.Y.) · 2016Article
- GATA1 Binding Kinetics on Conformation-Specific Binding Sites Elicit Differential Transcriptional Regulation.Molecular and cellular biology · 2016Article
- Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing.Nature immunology · 2016Article
- Article
- Whole-Body In Vivo Monitoring of Inflammatory Diseases Exploiting Human Interleukin 6-Luciferase Transgenic Mice.Molecular and cellular biology · 2015Article
- The Human GATA1 Gene Retains a 5' Insulator That Maintains Chromosomal Architecture and GATA1 Expression Levels in Splenic Erythroblasts.Molecular and cellular biology · 2015Article
- Progenitor stage-specific activity of a cis-acting double GATA motif for Gata1 gene expression.Molecular and cellular biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
GATA1 is a key regulator of erythroid cell differentiation. To examine how Gata1 gene expression is regulated in a stage-specific manner, transgenic mouse lines expressing green fluorescent protein (GFP) reporter from the Gata1 locus in a bacterial artificial chromosome (G1BAC-GFP) were prepared. We found that the GFP reporter expression faithfully recapitulated Gata1 gene expression. Using GFP fluorescence in combination with hematopoietic surface markers, we established a purification protocol for two erythroid progenitor fractions, referred to as burst-forming units-erythroid cell-related erythroid progenitor (BREP) and CFU-erythroid cell-related erythroid progenitor (CREP) fractions. We examined the functions of the Gata1 gene hematopoietic enhancer (G1HE) and the highly conserved GATA box in the enhancer core. Both deletion of the G1HE and substitution mutation of the GATA box caused almost complete loss of GFP expression in the BREP fraction, but the CREP stage expression was suppressed only partially, indicating the critical contribution of the GATA box to the BREP stage expression of Gata1. Consistently, targeted deletion of G1HE from the chromosomal Gata1 locus provoked suppressed expression of the Gata1 gene in the BREP fraction, which led to aberrant accumulation of BREP stage hematopoietic progenitor cells. These results demonstrate the physiological significance of the dynamic regulation of Gata1 gene expression in a differentiation stage-specific manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.