ArticleNucleic acids research2009
Structural analysis of DNA complexation with cationic lipids.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Restoration of p53 function via hybrid strontium sulfite nanoparticles modified LNP improves the antitumor efficacy in breast cancers.Cancer gene therapy · 2026Article
- Tunable Lipid Coatings Enable Cytoplasmic siRNA Delivery by DNA Origami.ACS applied materials & interfaces · 2026Article
- Ferrocene thiazolidine-2,4-dione derivatives cause DNA damage and interfere with DNA repair in triple-negative breast cancer cells.PloS one · 2025Article
- Prevention of prostate cancer metastasis by a CRISPR-delivering nanoplatform for interleukin-30 genome editing.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Lipid-Based Delivery Systems in Development of Genetic and Subunit Vaccines.Molecular biotechnology · 2023Review
- Mechanisms of Surfactin fromJournal of fungi (Basel, Switzerland) · 2023Article
- Interplay of the mechanical and structural properties of DNA nanostructures determines their electrostatic interactions with lipid membranes.Nanoscale · 2023Article
- A Surfactant Enables Efficient Membrane Spanning by Non-Aggregating DNA-Based Ion Channels.Molecules (Basel, Switzerland) · 2022Article
- Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery.Pharmaceutics · 2021Article
- Oligonucleotide Delivery across the Caco-2 Monolayer: The Design and Evaluation of Self-Emulsifying Drug Delivery Systems (SEDDS).Pharmaceutics · 2021Article
- Cationic vesicles for efficient shRNA transfection in the MCF-7 breast cancer cell line.International journal of nanomedicine · 2018Article
- MgJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Article
- Interaction of one anthraquinone derivative with ctDNA analyzed by spectroscopic and modeling methods.Journal of fluorescence · 2014Article
- A histone-like protein induces plasmid DNA to form liquid crystals in vitro and gene compaction in vivo.International journal of molecular sciences · 2013Article
- Gene transfection in high serum levels: case studies with new cholesterol based cationic gemini lipids.PloS one · 2013Article
- Physical factors affecting plasmid DNA compaction in stearylamine-containing nanoemulsions intended for gene delivery.Pharmaceuticals (Basel, Switzerland) · 2012Article
- Article
- Monitoring the reversible B to A-like transition of DNA in eukaryotic cells using Fourier transform infrared spectroscopy.Nucleic acids research · 2011Article
- Article
- Combinatorial approach to determine functional group effects on lipidoid-mediated siRNA delivery.Bioconjugate chemistry · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complexes of cationic liposomes with DNA are promising tools to deliver genetic information into cells for gene therapy and vaccines. Electrostatic interaction is thought to be the major force in lipid-DNA interaction, while lipid-base binding and the stability of cationic lipid-DNA complexes have been the subject of more debate in recent years. The aim of this study was to examine the complexation of calf-thymus DNA with cholesterol (Chol), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dioctadecyldimethylammoniumbromide (DDAB) and dioleoylphosphatidylethanolamine (DOPE), at physiological condition, using constant DNA concentration and various lipid contents. Fourier transform infrared (FTIR), UV-visible, circular dichroism spectroscopic methods and atomic force microscopy were used to analyse lipid-binding site, the binding constant and the effects of lipid interaction on DNA stability and conformation. Structural analysis showed a strong lipid-DNA interaction via major and minor grooves and the backbone phosphate group with overall binding constants of K(Chol) = 1.4 (+/-0.5) x 10(4) M(-1), K(DDAB) = 2.4 (+/-0.80) x 10(4) M(-1), K(DOTAP) = 3.1 (+/-0.90) x 10(4) M(-1) and K(DOPE) = 1.45 (+/- 0.60) x 10(4) M(-1). The order of stability of lipid-DNA complexation is DOTAP>DDAB>DOPE>Chol. Hydrophobic interactions between lipid aliphatic tails and DNA were observed. Chol and DOPE induced a partial B to A-DNA conformational transition, while a partial B to C-DNA alteration occurred for DDAB and DOTAP at high lipid concentrations. DNA aggregation was observed at high lipid content.
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