ArticleNucleic acids research2009
Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 41 citations in OpenAlex.
- The activity, divergence, and evolutionary degradation of modern-day homing endonucleases and behavior of their reconstructed ancestors.Nucleic acids research · 2026Article
- Halting Recombinant Adeno-Associated Virus Transgene Expression Using mRNA-Lipid Nanoparticle-Delivered Meganucleases.Human gene therapy · 2025Article
- Transgene removal using an in cis programmed homing endonuclease via single-strand annealing in the mosquito Aedes aegypti.Communications biology · 2024Article
- Precise cut-and-paste DNA insertion using engineered type V-K CRISPR-associated transposases.Nature biotechnology · 2023Article
- Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases.Nucleic acids research · 2018Article
- Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX).Nucleic acids research · 2017Article
- Massively parallel determination and modeling of endonuclease substrate specificity.Nucleic acids research · 2014Article
- Control of catalytic efficiency by a coevolving network of catalytic and noncatalytic residues.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Progressive engineering of a homing endonuclease genome editing reagent for the murine X-linked immunodeficiency locus.Nucleic acids research · 2014Article
- Bioinformatic analysis of the protein/DNA interface.Nucleic acids research · 2014Article
- Reprogramming homing endonuclease specificity through computational design and directed evolution.Nucleic acids research · 2014Article
- megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering.Nucleic acids research · 2014Article
- Novel fluorescent genome editing reporters for monitoring DNA repair pathway utilization at endonuclease-induced breaks.Nucleic acids research · 2014Article
- PARP-mediated repair, homologous recombination, and back-up non-homologous end joining-like repair of single-strand nicks.DNA repair · 2013Article
- Germline excision of transgenes in Aedes aegypti by homing endonucleases.Scientific reports · 2013Article
- Targeted DNA mutagenesis for the cure of chronic viral infections.Journal of virology · 2012Review
- Improved modeling of side-chain--base interactions and plasticity in protein--DNA interface design.Journal of molecular biology · 2012Article
- Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.Nucleic acids research · 2012Article
- Monomeric site-specific nucleases for genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Mining endonuclease cleavage determinants in genomic sequence data.The Journal of biological chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve endonuclease activity. The amino-acid substitutions increase target affinity, alter the thermal cleavage profile and significantly increase targeted recombination in transfected cells. The RNA splicing activity is not affected by these mutations. The improvement in DNA cleavage activity is largely focused on one of the enzyme's two active sites, corresponding to a rearrangement of a lysine residue hypothesized to act as a general base. Most of the constructs isolated in the screen contain one or more mutations that revert an amino-acid identity to a residue found in one or more close homologues of I-AniI. This implies that mutations that have previously reduced the endonuclease activity of I-AniI are identified and reversed, sometimes in combination with additional 'artificial' mutations, to optimize its in vivo activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.