ArticleThe Biochemical journal1991
Asymmetrical distribution of G-proteins among the apical and basolateral membranes of rat enterocytes.
Article in The Biochemical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Slc2a5 (Glut5) is essential for the absorption of fructose in the intestine and generation of fructose-induced hypertension.The Journal of biological chemistry · 2009Article
- Sodium and chloride absorptive defects in the small intestine in Slc26a6 null mice.Pflugers Archiv : European journal of physiology · 2008Article
- Down regulation of small intestinal ion transport in PDZK1- (CAP70/NHERF3) deficient mice.Pflugers Archiv : European journal of physiology · 2007Article
- Identification of guanine nucleotide binding regulatory proteins in bovine tracheal smooth muscle.Molecular and cellular biochemistry · 1996Article
- Complex information processing by the transmembrane signaling system involving G proteins.Naunyn-Schmiedeberg's archives of pharmacology · 1994Review
- Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells.The Journal of cell biology · 1993Article
- Multiple G-protein-dependent pathways mediate the antisecretory effects of somatostatin and clonidine in the HT29-19A colonic cell line.The Journal of clinical investigation · 1993Article
- Coupling of the alpha 2-adrenergic receptor to the inhibitory G-protein Gi and adenylate cyclase in HT29 cells.The Biochemical journal · 1993Article
- Characterization and distribution of alpha 2-adrenergic receptors in the human intestinal mucosa.The Journal of clinical investigation · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The distribution of the alpha and beta subunits of guanosine-nucleotide-binding proteins (G-proteins) among the apical and basolateral membranes of polarized rat enterocytes was investigated by ADP-ribosylation assays in vitro and immunoblotting with G-protein-subunit-specific antisera. The enterocytes were found to express alpha i2, alpha ji3, alpha s and beta subunits, whereas alpha i1 and alpha o subunits could not be detected. The alpha i2 and alpha i3 subunits were located predominantly in the basolateral membrane, in contrast with the alpha s and beta subunits, which were distributed uniformly among both membranes. Furthermore, 39 kDa and 78 kDa proteins, recognized by anti-alpha i1/2 but not anti-alpha i1 or anti-alpha i3 specific antisera, and resistant to ADP-ribosylation by pertussis toxin, were localized exclusively at the apical border. These Gi-related proteins might represent novel members of the G-protein family. Activation of apical G-proteins by GTP or its analogues failed to release the alpha s, alpha i and beta subunits or the 39 kDa and 78 kDa alpha i-like proteins from the membrane, suggesting a functional role for these proteins in the apical membrane itself. Our recent finding of a guanosine 5'-[gamma-thio]triphosphate-sensitive Cl- conductance in the apical membrane of rat enterocytes suggests that one or more of these G-proteins may act as local regulators of specific apical transport functions.
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