Evidence map›Paper›PMID 19101726›Full record

ArticleVirchows Archiv : an international journal of pathology2009

One-step nucleic acid amplification-a molecular method for the detection of lymph node metastases in breast cancer patients; results of the German study group.

Christian Schem, Nicolai Maass, Dirk O Bauerschlag, Martin H Carstensen, Thomas Löning, Christian Roder, Olivera Batic, Walter Jonat, Katharina Tiemann

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In one paragraph

Article in Virchows Archiv : an international journal of pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Trial
  5. Observational
  6. Article
  7. Review
  8. Micrometastasis in lymph nodes of colorectal cancer.Annals of gastroenterological surgery · 2022
    Review
  9. Article
  10. Article
  11. Predictive and prognostic value of total tumor load in sentinel lymph nodes in breast cancer patients after neoadjuvant treatment using one-step nucleic acid amplification: the NEOVATTL study.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021
    Article
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  15. One-step nucleic acid amplification (OSNA): where do we go with it?International journal of clinical oncology · 2017
    Review
  16. Article
  17. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Christian SchemDepartment of Obstetrics and Gynecology, University Clinic of Schleswig-Holstein, Campus Kiel, Michaelisstr. 16, 24105, Kiel, Germany. schemc@email.uni-kiel.de
Nicolai Maass
Dirk O Bauerschlag
Martin H Carstensen
Thomas Löning
Christian Roder
Olivera Batic
Walter Jonat
Katharina Tiemann

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sentinel lymph node (SN) biopsy is part of the staging procedure in breast cancer patients. In this study, we compared an intraoperative tool named one-step nucleic acid amplification (OSNA) to our routine histological investigation. OSNA consists of a short homogenization step followed by amplification of cytokeratin (CK) 19 mRNA directly from the lysate. To evaluate the performance of OSNA in comparison to histology, analysis of 343 axillary lymph nodes (ALN) from 93 breast cancer patients was performed with both methods. Discordant samples were subjected to other methods. If these tests supported the OSNA results, these samples were excluded from the study. The concordance rate was 91.8%, sensitivity 98.1%, and specificity 90.8% before and 95.5%, 100%, and 95.6%, respectively, after discordant case investigation. Our results show that OSNA is an excellent method for the detection of metastases in lymph nodes and can be applied as an intraoperative diagnostic approach.

Indexed as

Nucleic Acid Amplification TechniquesBreast NeoplasmsFemaleHumansKeratin-19Lymphatic MetastasisRNA, MessengerSentinel Lymph Node BiopsyKeratin-19RNA, Messenger

Identifiers

PMID19101726

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.