Evidence map›Paper›PMID 19100289›Full record

ArticleJournal of virological methods2009

A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24.

Angélique Biancotto, Beda Brichacek, Silvia S Chen, Wendy Fitzgerald, Andrea Lisco, Christophe Vanpouille, Leonid Margolis, Jean-Charles Grivel

Abstract read
In one paragraph

Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed.

  1. Article
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  6. Diagnostic applications of microsphere-based flow cytometry: A review.Experimental biology and medicine (Maywood, N.J.) · 2022
    Review
  7. Article
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  9. Article
  10. Extracellular Vesicles Generated by Gram-Positive Bacteria Protect Human TissuesFrontiers in cellular and infection microbiology · 2021
    Article
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Angélique BiancottoSection on Intercellular Interactions, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
Beda Brichacek
Silvia S Chen
Wendy Fitzgerald
Andrea Lisco
Christophe Vanpouille
Leonid Margolis
Jean-Charles Grivel

Funding

Cell Interactions in Three Dimensional Tissue CultureZIAHD001416 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI MARGOLIS, LEONID B. · 2009 to 2017
$15.7M
TISSUE IMAGING IN CELL BIOLOGYZ01HD001416 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI MARGOLIS, LEONID B. · 1993 to 2008
$2.7M
Intramural NIH HHS Z01 HD001416
6 · The paper itself

Abstract

Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current ELISA assays are expensive and offer a narrow dynamic measurement range. This report describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic measurement range. This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4pg to up to 20,000pgml(-1) in a volume of 50microl and can be combined with other measurements.

Indexed as

Flow CytometryFluorescent Antibody Technique, DirectHIV-1HIV Core Protein p24HumansMicrospheresSensitivity and SpecificityHIV Core Protein p24p24 protein, Human Immunodeficiency Virus Type 1

Identifiers

PMID19100289
PMCPMC4154487

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.