ArticleInfection and immunity1991
Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin.
Article in Infection and immunity, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 111 citations in OpenAlex.
- Article
- ThemBio · 2025Article
- RACK1 promotesiScience · 2023Article
- Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion.PLoS pathogens · 2023Article
- The type 3 secretion effector IpgD promotes S. flexneri dissemination.PLoS pathogens · 2022Article
- Article
- Rhes Tunnels: A Radical New Way of Communication in the Brain's Striatum?BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Review
- Mechanisms of bacillary dysentery: lessons learnt from infant rabbits.Gut microbes · 2020Review
- An Oral Inoculation Infant Rabbit Model formBio · 2020Article
- Critical role of bacterial dissemination in an infant rabbit model of bacillary dysentery.Nature communications · 2019Article
- Spatial, Temporal, and Functional Assessment of LC3-Dependent Autophagy in Shigella flexneri Dissemination.Infection and immunity · 2018Article
- Actin-based motility of bacterial pathogens: mechanistic diversity and its impact on virulence.Pathogens and disease · 2016Article
- Article
- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- Bacterial spread from cell to cell: beyond actin-based motility.Trends in microbiology · 2015Review
- The class II phosphatidylinositol 3-phosphate kinase PIK3C2A promotes Shigella flexneri dissemination through formation of vacuole-like protrusions.Infection and immunity · 2015Article
- Article
- Ethanol suppresses phagosomal adhesion maturation, Rac activation, and subsequent actin polymerization during FcγR-mediated phagocytosis.Cellular immunology · 2012Article
- Outer membrane protein A (OmpA): a new player in shigella flexneri protrusion formation and inter-cellular spreading.PloS one · 2012Article
- Pathogens and polymers: microbe-host interactions illuminate the cytoskeleton.The Journal of cell biology · 2011Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spread of Shigella flexneri in a monolayer of infected Henle and HeLa cells was studied by using immunofluorescence and electron microscopy. Infected cells produced numerous bacterium-containing membranous protrusions up to 18 microns in length that penetrated adjacent cells and were subsequently phagocytosed. Fluorescence staining of actin and vinculin in infected cells with phalloidin and monoclonal antibody to vinculin, respectively, demonstrated that the protrusions containing the bacteria consisted of these cytoskeletal proteins. Actin accumulated predominantly at the poles of bacteria distal to the tip of protrusions and appeared as trails extending back towards the host cell cytoplasm. Vinculin, however, was distributed uniformly around the bacteria and throughout the protrusion. A profound rearrangement of vinculin occurred in Henle and HeLa cells following infection with shigellae: whereas in uninfected cells it was distributed mainly around the cell periphery, in infected cells it concentrated mainly around clusters of bacteria in the cytoplasm. This suggests a possible involvement of the vinculin cytoskeletal protein in the intercellular spread of shigellae during an infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.