ArticleInvestigative ophthalmology & visual science2009
Potential for transcriptional upregulation of cochlin in glaucomatous trabecular meshwork: a combinatorial bioinformatic and biochemical analytical approach.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Immunohistochemical localization of Nrf2 in the human cochlea.Brain research · 2018Article
- Cochlin in normal middle ear and abnormal middle ear deposits in DFNA9 and Coch (G88E/G88E) mice.Journal of the Association for Research in Otolaryngology : JARO · 2014Article
- Review of application of mass spectrometry for analyses of anterior eye proteome.World journal of biological chemistry · 2014Review
- Analysis of COCH and TNFA variants in East Indian primary open-angle glaucoma patients.BioMed research international · 2013Article
- Anterior segment alterations and comparative aqueous humor proteomics in the buphthalmic rabbit (an American Ophthalmological Society thesis).Transactions of the American Ophthalmological Society · 2011Review
- Extralabyrinthine manifestations of DFNA9.Journal of the Association for Research in Otolaryngology : JARO · 2011Article
- Expression of caveolin in trabecular meshwork cells and its possible implication in pathogenesis of primary open angle glaucoma.Molecular vision · 2011Article
- Proteomics, bioinformatics and targeted gene expression analysis reveals up-regulation of cochlin and identifies other potential biomarkers in the mouse model for deafness in Usher syndrome type 1F.Human molecular genetics · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine the existence of a relatively higher abundance of potential TFs in glaucomatous trabecular meshwork (TM) that may bind putative promoter regions and affect cochlin protein expression in glaucomatous compared to normal TM.
methodsCombinatorial bioinformatics and biochemical analyses, using human glaucomatous and normal donor tissue (n = 4 each). Biochemical analysis included electrophoretic mobility shift assays (EMSAs), filter binding assays (FBAs), coupled in vitro transcription-translation (TNT) assays and promoter mutation analysis.
resultsCombinatorial bioinformatics and biochemical analyses revealed the existence of a higher abundance of TFs in glaucomatous than in normal TM nuclear extracts. The evidence of a relatively high abundance of TFs, leading to increased expression of cochlin predicted by bioinformatic and biochemical analyses (EMSA and FBA), was further supported by TNT and promoter mutation TNT assays.
conclusionsThese results support the finding that the observed increased cochlin expression in glaucomatous TM is due to relative elevated abundance of TFs. The results also demonstrate the utility of combinatorial bioinformatic and biochemical analyses for genes with uncharacterized promoter regions.
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