ArticleThe Plant cell2008
Characterization of Raphanus sativus pentatricopeptide repeat proteins encoded by the fertility restorer locus for Ogura cytoplasmic male sterility.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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Who cites it
79 citing papers in PubMed, 158 citations in OpenAlex.
- Mitochondrial-Nuclear Interactions, Co-Transcription, and Adaptive Evolution in Cytoplasmic Male Sterility.Plant, cell & environment · 2026Review
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- A PPR gene cluster from Solanum aethiopicum underlies fertility restoration in cytoplasmic male-sterile eggplant (S. melongena L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Genetic Resources of Cereal and Oilseed Crops for Heterotic Hybrid Breeding.Plants (Basel, Switzerland) · 2025Review
- The chimeric gene orf610a reduces cotton pollen fertility by impairing the assembly of ATP synthase.Plant biotechnology journal · 2025Article
- Pentatricopeptide repeat proteins in plants: Cellular functions, action mechanisms, and potential applications.Plant communications · 2025Review
- Chromosome-level assemblies of Amaranthus palmeri, Amaranthus retroflexus, and Amaranthus hybridus allow for genomic comparisons and identification of a sex-determining region.The Plant journal : for cell and molecular biology · 2025Article
- Analysis ofInternational journal of molecular sciences · 2024Article
- Fertility restorer geneHorticulture research · 2024Article
- Review
- Article
- Cytological and Molecular Characterization of a New Ogura Cytoplasmic Male Sterility Restorer ofPlants (Basel, Switzerland) · 2024Article
- A P-type pentatricopeptide repeat protein ZmRF5 promotes 5' region partial cleavages of atp6c transcripts to restore the fertility of CMS-C maize by recruiting a splicing factor.Plant biotechnology journal · 2024Article
- Editing of ORF138 restores fertility of Ogura cytoplasmic male sterile broccoli via mitoTALENs.Plant biotechnology journal · 2024Article
- Genome-Wide Identification of the SPP/SPPL Gene Family andInternational journal of molecular sciences · 2024Article
- A breeding method for Ogura CMS restorer line independent of restorer source inFrontiers in genetics · 2024Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Genomic profiling of dioecious Amaranthus species provides novel insights into species relatedness and sex genes.BMC biology · 2023Article
- Induction of Male Sterility by Targeted Mutation of a Restorer-of-Fertility Gene with CRISPR/Cas9-Mediated Genome Editing inPlants (Basel, Switzerland) · 2022Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic male sterility is a maternally inherited trait in higher plants that prevents the production of functional pollen. Ogura cytoplasmic male sterility in radish (Raphanus sativus) is regulated by the orf138 mitochondrial locus. Male fertility can be restored when orf138 accumulation is suppressed by the nuclear Rfo locus, which consists of three genes putatively encoding highly similar pentatricopeptide repeat proteins (PPR-A, -B, and -C). We produced transgenic rapeseed (Brassica napus) plants separately expressing PPR-A and PPR-B and demonstrated that both encoded proteins accumulated preferentially in the anthers of young flower buds. Immunodetection of ORF138 showed that, unlike PPR-B, PPR-A had no effect on the synthesis of the sterility protein. Moreover, immunolocalization experiments indicated that complete elimination of ORF138 from the tapetum of anthers correlated with the restoration of fertility. Thus, the primary role of PPR-B in restoring fertility is to inhibit ORF138 synthesis in the tapetum of young anthers. In situ hybridization experiments confirmed, at the cellular level, that PPR-B has no effect on the accumulation of orf138 mRNA. Lastly, immunoprecipitation experiments demonstrated that PPR-B, but not PPR-A, is associated with the orf138 RNA in vivo, linking restoration activity with the ability to directly or indirectly interact with the orf138 RNA. Together, our data support a role for PPR-B in the translational regulation of orf138 mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.