ArticlePlant physiology2009
A pollen protein, NaPCCP, that binds pistil arabinogalactan proteins also binds phosphatidylinositol 3-phosphate and associates with the pollen tube endomembrane system.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- OsVPS34-generated PI3P recruits GPA5/Rab5a to regulate post-Golgi glutelin trafficking in rice endosperm.Plant physiology · 2026Article
- Article
- Identifying Genome-Wide Sequence Variations and Candidate Genes Implicated in Self-Incompatibility by ResequencingInternational journal of molecular sciences · 2019Article
- The unique evolutionary pattern of the Hydroxyproline-rich glycoproteins superfamily in Chinese white pear (Pyrus bretschneideri).BMC plant biology · 2018Article
- Gene expression of an arabinogalactan lysine-rich protein3 Biotech · 2018Article
- Floral Metabolism of Sugars and Amino Acids: Implications for Pollinators' Preferences and Seed and Fruit Set.Plant physiology · 2017Review
- SIPP, a Novel Mitochondrial Phosphate Carrier, Mediates in Self-Incompatibility.Plant physiology · 2017Article
- Genomic, Molecular Evolution, and Expression Analysis of Genes Encoding Putative Classical AGPs, Lysine-Rich AGPs, and AG Peptides inFrontiers in plant science · 2017Article
- Importance of organellar proteins, protein translocation and vesicle transport routes for pollen development and function.Plant reproduction · 2016Review
- Male functions and malfunctions: the impact of phosphoinositides on pollen development and pollen tube growth.Plant reproduction · 2016Review
- Arabinogalactan proteins: rising attention from plant biologists.Plant reproduction · 2015Review
- Emerging roles for microtubules in angiosperm pollen tube growth highlight new research cues.Frontiers in plant science · 2015Article
- BcMF8, a putative arabinogalactan protein-encoding gene, contributes to pollen wall development, aperture formation and pollen tube growth in Brassica campestris.Annals of botany · 2014Article
- NaStEP: a proteinase inhibitor essential to self-incompatibility and a positive regulator of HT-B stability in Nicotiana alata pollen tubes.Plant physiology · 2013Article
- Identification of differentially expressed genes in pistils from self-incompatible Citrus reticulata by suppression subtractive hybridization.Molecular biology reports · 2013Article
- Arabinogalactan-protein secretion is associated with the acquisition of stigmatic receptivity in the apple flower.Annals of botany · 2012Article
- Arabinogalactan proteins in root and pollen-tube cells: distribution and functional aspects.Annals of botany · 2012Review
- Physical mapping of a pollen modifier locus controlling self-incompatibility in apricot and synteny analysis within the Rosaceae.Plant molecular biology · 2012Article
- Interspecific reproductive barriers in the tomato clade: opportunities to decipher mechanisms of reproductive isolation.Sexual plant reproduction · 2011Review
- Compatibility and incompatibility in S-RNase-based systems.Annals of botany · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As pollen tubes grow toward the ovary, they are in constant contact with the pistil extracellular matrix (ECM). ECM components are taken up during growth, and some pistil molecules exert their effect inside the pollen tube. For instance, the Nicotiana alata 120-kD glycoprotein (120K) is an abundant arabinogalactan protein that is taken up from the ECM; it has been detected in association with pollen tube vacuoles, but the transport pathway between these compartments is unknown. We recently identified a pollen C2 domain-containing protein (NaPCCP) that binds to the carboxyl-terminal domain of 120K. As C2 domain proteins mediate protein-lipid interactions, NaPCCP could function in intracellular transport of 120K in pollen tubes. Here, we describe binding studies showing that the NaPCCP C2 domain is functional and that binding is specific for phosphatidylinositol 3-phosphate. Subcellular fractionation, immunolocalization, and live imaging results show that NaPCCP is associated with the plasma membrane and internal pollen tube vesicles. Colocalization between an NaPCCPgreen fluorescent protein fusion and internalized FM4-64 suggest an association with the endosomal system. NaPCCP localization is altered in pollen tubes rejected by the self-incompatibility mechanism, but our hypothesis is that it has a general function in the transport of endocytic cargo rather than a specific function in self-incompatibility. NaPCCP represents a bifunctional protein with both phosphatidylinositol 3-phosphate- and arabinogalactan protein-binding domains. Therefore, it could function in the transport of pistil ECM proteins in the pollen tube endomembrane system.
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