ArticlePlant physiology2009
Multidrug and toxic compound extrusion-type transporters implicated in vacuolar sequestration of nicotine in tobacco roots.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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101 citing papers in PubMed, 228 citations in OpenAlex.
- Characterization of Nucleobase Cation Symporter 1 (NCS1) Localized to the Plastids of Nicotiana tabacum.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- A sweet solution to nicotine biosynthesis.Nature plants · 2026Article
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- Nicotine biosynthesis is completed by cryptic activating glucosylation.Nature communications · 2026Article
- Auxin-Induced Nicotine Inhibition Is Mediated by NaARF5 Through the Suppression of NaERF1-Like Expression and Interaction With NaERF1-Like in Nicotiana attenuata.Plant biotechnology journal · 2026Article
- Integrated transcriptomic and metabolomic analyses reveal the regulatory network underlying NtGSTU10-mediated nicotine synthesis and transport in tobacco.Scientific reports · 2026Article
- Identification and functional analysis of a gene regulating tolerance to freezing in Brassica Napus L. seedlings using BSA-seq and RNA-seq.BMC plant biology · 2025Article
- Transport of secondary metabolites in plants: Mechanistic insights and transporter engineering for crop improvement.Plant communications · 2025Review
- Overexpression of NtGSTU10 gene enhances nicotine content in hybrids by promoting the synthesis and transport metabolism of nicotine.BMC biotechnology · 2025Article
- Structural insights into a citrate transporter that mediates aluminum tolerance in barley.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- PdMATE16 and PdMATE35 are essential for the accumulation of multiple flavonoids in tree peonies.Frontiers in plant science · 2025Article
- Transportation engineering for enhanced production of plant natural products in microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Multidrug and Toxic Compound Extrusion Transporters: Ubiquitous Multifaceted Proteins in Microbes, Plants, and Their Interactions.Microorganisms · 2024Review
- The formation of nicotine heterosis is mainly achieved by enhancing the nicotine transport capacity in hybrids.BMC plant biology · 2024Article
- Integration of co-culture and transport engineering for enhanced metabolite production.Plant biotechnology (Tokyo, Japan) · 2024Article
- Characterization of a vacuolar importer of secologanin in Catharanthus roseus.Communications biology · 2024Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- The leaf idioblastome of the medicinal plant Catharanthus roseus is associated with stress resistance and alkaloid metabolism.Journal of experimental botany · 2024Article
- Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.).BMC genomics · 2023Article
- Linking plant functional genes to rhizosphere microbes: a review.Plant biotechnology journal · 2023Review
41 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotine is a major alkaloid accumulating in the vacuole of tobacco (Nicotiana tabacum), but the transporters involved in the vacuolar sequestration are not known. We here report that tobacco genes (NtMATE1 and NtMATE2) encoding transporters of the multidrug and toxic compound extrusion (MATE) family are coordinately regulated with structural genes for nicotine biosynthesis in the root, with respect to spatial expression patterns, regulation by NIC regulatory loci, and induction by methyl jasmonate. Subcellular fractionation, immunogold electron microscopy, and expression of a green fluorescent protein fusion protein all suggested that these transporters are localized to the vacuolar membrane. Reduced expression of the transporters rendered tobacco plants more sensitive to the application of nicotine. In contrast, overexpression of NtMATE1 in cultured tobacco cells induced strong acidification of the cytoplasm after jasmonate elicitation or after the addition of nicotine under nonelicited conditions. Expression of NtMATE1 in yeast (Saccharomyces cerevisiae) cells compromised the accumulation of exogenously supplied nicotine into the yeast cells. The results imply that these MATE-type proteins transport tobacco alkaloids from the cytosol into the vacuole in exchange for protons in alkaloid-synthesizing root cells.
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