ArticleThe Journal of biological chemistry2009
Molecular mechanisms of yeast cell wall glucan remodeling.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Who cites it
31 citing papers in PubMed, 88 citations in OpenAlex.
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- Preparation of Yeast Extract from Brewer's Yeast Waste and Its Potential Application as a Medium Constituent.Applied biochemistry and biotechnology · 2024Article
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- Cell envelope and stress-responsive pathways underlie an evolved oleaginous Rhodotorula toruloides strain multi-stress tolerance.Biotechnology for biofuels and bioproducts · 2024Article
- Synergistic Antifungal Interactions between Antibiotic Amphotericin B and SelectedInternational journal of molecular sciences · 2023Article
- Role of combined cell membrane and wall mechanical properties regulated by polarity signals in cell budding.Physical biology · 2020Article
- Antifungal effects of a 1,3,4-thiadiazole derivative determined by cytochemical and vibrational spectroscopic studies.PloS one · 2019Article
- The Genetics and Biochemistry of Cell Wall Structure and Synthesis inFrontiers in microbiology · 2019Review
- Cell wall polysaccharides: before and after autolysis of brewer's yeast.World journal of microbiology & biotechnology · 2018Review
- Gene Expression of Pneumocystis murina after Treatment with Anidulafungin Results in Strong Signals for Sexual Reproduction, Cell Wall Integrity, and Cell Cycle Arrest, Indicating a Requirement for Ascus Formation for Proliferation.Antimicrobial agents and chemotherapy · 2018Article
- The PHR Family: The Role of Extracellular Transglycosylases in Shaping Candida albicans Cells.Journal of fungi (Basel, Switzerland) · 2017Review
- The Fungal Cell Wall: Structure, Biosynthesis, and Function.Microbiology spectrum · 2017Review
- Genetic and biochemical characterization of the GH72 family of cell wall transglycosylases in Neurospora crassa.Fungal genetics and biology : FG & B · 2017Article
- The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection.Cellular microbiology · 2017Article
- Characterization of the Sclerotinia sclerotiorum cell wall proteome.Molecular plant pathology · 2016Article
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- Validation of Reference Genes for Robust qRT-PCR Gene Expression Analysis in the Rice Blast Fungus Magnaporthe oryzae.PloS one · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Yeast cell wall remodeling is controlled by the equilibrium between glycoside hydrolases, glycosyltransferases, and transglycosylases. Family 72 glycoside hydrolases (GH72) are ubiquitous in fungal organisms and are known to possess significant transglycosylase activity, producing elongated beta(1-3) glucan chains. However, the molecular mechanisms that control the balance between hydrolysis and transglycosylation in these enzymes are not understood. Here we present the first crystal structure of a glucan transglycosylase, Saccharomyces cerevisiae Gas2 (ScGas2), revealing a multidomain fold, with a (betaalpha)(8) catalytic core and a separate glucan binding domain with an elongated, conserved glucan binding groove. Structures of ScGas2 complexes with different beta-glucan substrate/product oligosaccharides provide "snapshots" of substrate binding and hydrolysis/transglycosylation giving the first insights into the mechanisms these enzymes employ to drive beta(1-3) glucan elongation. Together with mutagenesis and analysis of reaction products, the structures suggest a "base occlusion" mechanism through which these enzymes protect the covalent protein-enzyme intermediate from a water nucleophile, thus controlling the balance between hydrolysis and transglycosylation and driving the elongation of beta(1-3) glucan chains in the yeast cell wall.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.