ArticlePLoS genetics2008
Topoisomerase 3alpha and RMI1 suppress somatic crossovers and are essential for resolution of meiotic recombination intermediates in Arabidopsis thaliana.
Article in PLoS genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 92 citations in OpenAlex.
- ZEP1 orchestrates template choice and crossover pathways to ensure meiotic genome integrity in rice.The EMBO journal · 2026Article
- RMI2 regulates recombination intermediate metabolism to ensure proper chromosome segregation in mammalian meiosis.Nucleic acids research · 2026Article
- The genomic landscape of recombination in rice revealed by a large nested association mapping population.The New phytologist · 2026Article
- The post-fertilization archegonium gene network of seedless plants contributed to the origin of seeds.Nature communications · 2026Article
- Transcription factor-promoter interactions forbioRxiv : the preprint server for biology · 2025Article
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- Complex Polyploids: Origins, Genomic Composition, and Role of Introgressed Alleles.Systematic biology · 2024Article
- Formation and resolution of meiotic chromosome entanglements and interlocks.Journal of cell science · 2024Review
- Divergence and conservation of the meiotic recombination machinery.Nature reviews. Genetics · 2024Review
- KNO1-mediated autophagic degradation of the Bloom syndrome complex component RMI1 promotes homologous recombination.The EMBO journal · 2023Article
- Technology-driven approaches for meiosis research in tomato and wild relatives.Plant reproduction · 2023Article
- Crossover interference mechanism: New lessons from plants.Frontiers in cell and developmental biology · 2023Review
- Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in SyntheticPlants (Basel, Switzerland) · 2022Article
- The topoisomerase 3 zinc finger domain cooperates with the RMI1 scaffold to promote stable association of the BTR complex to recombination intermediates in the Caenorhabditis elegans germline.Nucleic acids research · 2022Article
- Holliday junction resolution by At-HIGLE: an SLX1 lineage endonuclease from Arabidopsis thaliana with a novel in-built regulatory mechanism.Nucleic acids research · 2022Article
- Meiosis in crops: from genes to genomes.Journal of experimental botany · 2021Review
- Caenorhabditis elegans RMI2 functional homolog-2 (RMIF-2) and RMI1 (RMH-1) have both overlapping and distinct meiotic functions within the BTR complex.PLoS genetics · 2021Article
- Fanconi anemia ortholog FANCM regulates meiotic crossover distribution in plants.Plant physiology · 2021Article
- CRISPR-Cas-mediated chromosome engineering for crop improvement and synthetic biology.Nature plants · 2021Review
- From Microscopy to Nanoscopy: Defining anFrontiers in plant science · 2021Review
11 more citing papers are in PubMed but not listed here.
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Topoisomerases are enzymes with crucial functions in DNA metabolism. They are ubiquitously present in prokaryotes and eukaryotes and modify the steady-state level of DNA supercoiling. Biochemical analyses indicate that Topoisomerase 3alpha (TOP3alpha) functions together with a RecQ DNA helicase and a third partner, RMI1/BLAP75, in the resolution step of homologous recombination in a process called Holliday Junction dissolution in eukaryotes. Apart from that, little is known about the role of TOP3alpha in higher eukaryotes, as knockout mutants show early lethality or strong developmental defects. Using a hypomorphic insertion mutant of Arabidopsis thaliana (top3alpha-2), which is viable but completely sterile, we were able to define three different functions of the protein in mitosis and meiosis. The top3alpha-2 line exhibits fragmented chromosomes during mitosis and sensitivity to camptothecin, suggesting an important role in chromosome segregation partly overlapping with that of type IB topoisomerases. Furthermore, AtTOP3alpha, together with AtRECQ4A and AtRMI1, is involved in the suppression of crossover recombination in somatic cells as well as DNA repair in both mammals and A. thaliana. Surprisingly, AtTOP3alpha is also essential for meiosis. The phenotype of chromosome fragmentation, bridges, and telophase I arrest can be suppressed by AtSPO11 and AtRAD51 mutations, indicating that the protein is required for the resolution of recombination intermediates. As Atrmi1 mutants have a similar meiotic phenotype to Attop3alpha mutants, both proteins seem to be involved in a mechanism safeguarding the entangling of homologous chromosomes during meiosis. The requirement of AtTOP3alpha and AtRMI1 in a late step of meiotic recombination strongly hints at the possibility that the dissolution of double Holliday Junctions via a hemicatenane intermediate is indeed an indispensable step of meiotic recombination.
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