ArticleCell death and differentiation2009
Polarized fluid movement and not cell death, creates luminal spaces in adult prostate epithelium.
Article in Cell death and differentiation, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- The Mammary Gland: Basic Structure and Molecular Signaling during Development.International journal of molecular sciences · 2022Review
- Osmotic Gradients in Epithelial Acini Increase Mechanical Tension across E-cadherin, Drive Morphogenesis, and Maintain Homeostasis.Current biology : CB · 2020Article
- Arsenite malignantly transforms human prostate epithelial cells in vitro by gene amplification of mutated KRAS.PloS one · 2019Article
- MCF-7 Human Breast Cancer Cells Form Differentiated Microtissues in Scaffold-Free Hydrogels.PloS one · 2015Article
- DNA hypermethylation in prostate cancer is a consequence of aberrant epithelial differentiation and hyperproliferation.Cell death and differentiation · 2014Article
- An integrin-ILK-microtubule network orients cell polarity and lumen formation in glandular epithelium.Nature cell biology · 2013Article
- Article
- Stromal upregulation of lateral epithelial adhesions: gene expression analysis of signalling pathways in prostate epithelium.Journal of biomedical science · 2011Article
- Stroma regulates increased epithelial lateral cell adhesion in 3D culture: a role for actin/cadherin dynamics.PloS one · 2011Article
- Molecular regulation of lumen morphogenesis.Current biology : CB · 2011Review
- Disruption of a Sirt1-dependent autophagy checkpoint in the prostate results in prostatic intraepithelial neoplasia lesion formation.Cancer research · 2011Article
- Cell-matrix interactions in mammary gland development and breast cancer.Cold Spring Harbor perspectives in biology · 2010Review
- Modeling the prostate stem cell niche: an evaluation of stem cell survival and expansion in vitro.Stem cells and development · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
There are two predominant theories for lumen formation in tissue morphogenesis: cavitation driven by cell death, and membrane separation driven by epithelial polarity. To define the mechanism of lumen formation in prostate acini, we examined both theories in several cell lines grown in three-dimensional (3D) Matrigel culture. Lumen formation occurred early in culture and preceded the expression of cell death markers for apoptosis (active caspase 3) and autophagy (LC-3). Active caspase 3 was expressed by very few cells and inhibition of apoptosis did not suppress lumen formation. Despite LC-3 expression in all cells within a spheroid, this was not associated with cell death. However, expression of a prostate-secretory protein coincided with lumen formation and subsequent disruption of polarized fluid movement led to significant inhibition of lumen formation. This work indicates that lumen formation is driven by the polarized movement of fluids and proteins in 3D prostate epithelial models and not by cavitation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.