ArticleRestorative neurology and neuroscience2008
Dopaminergic neurons derived from BG01V2, a variant of human embryonic stem cell line BG01.
Article in Restorative neurology and neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Functional consequences of 17q21.31/WNT3-WNT9B amplification in hPSCs with respect to neural differentiation.Cell reports · 2015Article
- Developmental incompatibility of human parthenogenetic embryonic stem cells in mouse blastocysts.In vitro cellular & developmental biology. Animal · 2012Article
- Article
- A novel combination of factors, termed SPIE, which promotes dopaminergic neuron differentiation from human embryonic stem cells.PloS one · 2009Article
- Human embryonic stem cells which express hrGFP in the undifferentiated state and during dopaminergic differentiation.Restorative neurology and neuroscience · 2009Article
- Important precautions when deriving patient-specific neural elements from pluripotent cells.Cytotherapy · 2009Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
background and purposeHuman embryonic stem cells (hESC) are considered a renewable source of dopamine producing neurons, and are of particular interest for their potential clinical use in Parkinson's disease. In this study, we characterized human dopaminergic neurons generated by stromal-derived inducing activity (SDIA) from BG01V2, a strain of human embryonic stem cell line, BG01, characterized by a chromosome 17 trisomy. Similar chromosomal changes have been repeatedly observed in hESC cultures in different laboratories, indicating the importance of chromosome 17 for growth and adaptation of hESC to culture.
methodsWe investigated in vitro proliferation of differentiating cells using a BrDU incorporation assay, and monitored the cell population in long term cultures. Despite the cytogenetic abnormality, TH+ neurons were postmitotic at all stages of differentiation. After 30 days of differentiation, cell division ceased in 91% of the overall population of cells in the culture, indicating intact cell cycle regulation.
resultsExpression of midbrain specific marker genes (Otx2, Pax5, Msx-1) showed differentiation of hESC-derived neural progenitor cells into midbrain specific dopamine neurons. These neurons expressed the dopamine transporter (DAT), and displayed functional DAT activity and electrical excitability.
conclusionsTH+ cells derived from the BG01V2 hESC line using SDIA are postmitotic and have functional characteristics of normal dopaminergic neurons.
Indexed as
Identifiers
19096132PMC2696893W74297105What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.