ArticleJournal of lipid research2009
Identification of phosphatidylserylglutamate: a novel minor lipid in Escherichia coli.
Article in Journal of lipid research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Localization of cyclopropyl groups and alkenes within glycerophospholipids using gas-phase ion/ion chemistry.Journal of mass spectrometry : JMS · 2023Article
- Transient Complexity ofMetabolites · 2022Article
- Substrate Specificities and Efflux Efficiencies of RND Efflux Pumps of Acinetobacter baumannii.Journal of bacteriology · 2018Article
- Does triacylglycerol (TAG) serve a photoprotective function in plant leaves? An examination of leaf lipids under shading and drought.Physiologia plantarum · 2017Article
- Altered Lipid Synthesis by Lack of Yeast Pah1 Phosphatidate Phosphatase Reduces Chronological Life Span.The Journal of biological chemistry · 2015Article
- Non-enzymatically derived minor lipids found in Escherichia coli lipid extracts.Biochimica et biophysica acta · 2011Article
- Putative N-acylphosphatidylethanolamine synthase from Arabidopsis thaliana is a lysoglycerophospholipid acyltransferase.The Journal of biological chemistry · 2011Article
- Applications of mass spectrometry to lipids and membranes.Annual review of biochemistry · 2011Review
- The glycerophospho metabolome and its influence on amino acid homeostasis revealed by brain metabolomics of GDE1(-/-) mice.Chemistry & biology · 2010Article
- Lipidomics: a mass spectrometry based systems level analysis of cellular lipids.Current opinion in chemical biology · 2009Review
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Authors and funding
6 authors.
Funding
Abstract
Advances in mass spectrometry have facilitated the identification of novel lipid structures. In this work, we fractionated the lipids of Escherichia coli B and analyzed the fractions using negative-ion electrospray ionization mass spectrometry to reveal unknown lipid structures. Analysis of a fraction eluting with high salt from DEAE cellulose revealed a series of ions not corresponding to any of the known lipids of E. coli. The ions, with m/z 861.5, 875.5, 887.5, 889.5, and 915.5, were analyzed using collision-induced dissociation mass spectrometry (MS/MS) and yielded related fragmentation patterns consistent with a novel diacylated glycerophospholipid. Product ions arising by neutral loss of 216 mass units were observed with all of the unknowns. A corresponding negative product ion was also observed at m/z 215.0. Additional ions at m/z 197.0, 171.0, 146.0, and 128.0 were used to propose the novel structure phosphatidylserylglutamate (PSE). The hypothesized structure was confirmed by comparison with the MS/MS spectrum of a synthetic standard. Normal phase liquid chromatography-mass spectrometry analysis further showed that the endogenous PSE and synthetic PSE eluted with the same retention times. PSE was also observed in the equivalent anion exchange fractions of total lipids extracted from the wild-type E. coli K-12 strain MG1655.
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