ArticleRNA (New York, N.Y.)2009
The MRB1 complex functions in kinetoplastid RNA processing.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 52 citations in OpenAlex.
- Structural basis for guide RNA selection by the RESC1-RESC2 complex.Nucleic acids research · 2023Article
- Trypanosome RNAEditing Substrate Binding Complex integrity and function depends on the upstream action of RESC10.Nucleic acids research · 2021Article
- Complexome Profiling-Exploring Mitochondrial Protein Complexes in Health and Disease.Frontiers in cell and developmental biology · 2021Review
- Developmental regulation of edited CYb and COIII mitochondrial mRNAs is achieved by distinct mechanisms in Trypanosoma brucei.Nucleic acids research · 2020Article
- Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.Trends in parasitology · 2020Review
- Cell-line specific RNA editing patterns in Trypanosoma brucei suggest a unique mechanism to generate protein variation in a system intolerant to genetic mutations.Nucleic acids research · 2020Article
- MRB10130 is a RESC assembly factor that promotes kinetoplastid RNA editing initiation and progression.RNA (New York, N.Y.) · 2019Article
- Intrinsic and regulated properties of minimally edited trypanosome mRNAs.Nucleic acids research · 2019Article
- The RRM of the kRNA-editing protein TbRGG2 uses multiple surfaces to bind and remodel RNA.Nucleic acids research · 2019Article
- Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes.RNA (New York, N.Y.) · 2018Article
- MRB7260 is essential for productive protein-RNA interactions within the RNA editing substrate binding complex during trypanosome RNA editing.RNA (New York, N.Y.) · 2018Article
- The essential functions of KREPB4 are developmentally distinct and required for endonuclease association with editosomes.RNA (New York, N.Y.) · 2017Article
- High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing.RNA (New York, N.Y.) · 2016Article
- REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes.The Journal of biological chemistry · 2016Article
- A Protein Complex Map of Trypanosoma brucei.PLoS neglected tropical diseases · 2016Article
- U-Insertion/Deletion mRNA-Editing Holoenzyme: Definition in Sight.Trends in parasitology · 2016Review
- Integrity of the core mitochondrial RNA-binding complex 1 is vital for trypanosome RNA editing.RNA (New York, N.Y.) · 2015Article
- Structures of the T. brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold.Nucleic acids research · 2015Article
- Bloodstream form Trypanosoma brucei do not require mRPN1 for gRNA processing.RNA (New York, N.Y.) · 2015Article
- RNA binding and core complexes constitute the U-insertion/deletion editosome.Molecular and cellular biology · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mitochondrial (mt) gene expression in Trypanosoma brucei entails multiple types of RNA processing, including polycistronic transcript cleavage, mRNA editing, gRNA oligouridylation, and mRNA polyadenylation, which are catalyzed by various multiprotein complexes. We examined the novel mitochondrial RNA-binding 1 (MRB1) complex that has 16 associated proteins, four of which have motifs suggesting RNA interaction. RNase treatment or the lack of kDNA in mutants resulted in lower MRB1 complex sedimentation in gradients, indicating that MRB1 complex associates with kDNA transcripts. RNAi knockdowns of expression of the Tb10.406.0050 (TbRGGm, RGG motif), Tb927.6.1680 (C2H2 zinc finger), and Tb11.02.5390 (no known motif) MRB1 proteins each inhibited in vitro growth of procyclic form parasites and resulted in cells with abnormal numbers of nuclei. Knockdown of TbRGGm, but not the other two proteins, disrupted the MRB1 complex, indicating that it, but perhaps not the other two, is required for complex assembly and/or stability. The knockdowns resulted in similar but nonidentical patterns of altered in vivo abundances of edited, pre-edited, and preprocessed mt mRNAs, but did not appreciably affect the abundances of mRNAs that do not get edited. These results indicate that MRB1 complex is critical to the processing of mt RNAs, and although its specific function is unknown, it appears essential to parasite viability.
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