ArticleThe Journal of molecular diagnostics : JMD2009
Multi-probe real-time PCR identification of common Mycobacterium species in blood culture broth.
Article in The Journal of molecular diagnostics : JMD, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Diagnostic accuracy of interferon-gamma release assay and mucosal-associated invariant T cells in spinal tuberculosis.Scientific reports · 2025Article
- Cutaneous Infection Caused byHealth science reports · 2025Article
- Optimized Method for Bacterial Nucleic Acid Extraction from Positive Blood Culture Broth for Whole-Genome Sequencing, Resistance Phenotype Prediction, and Downstream Molecular Applications.Journal of clinical microbiology · 2022Article
- Characterization of MicroRNA Expression Profiles and Identification of Potential Biomarkers in Leprosy.Journal of clinical microbiology · 2017Article
- Drug Susceptibility of 33 Reference Strains of Slowly Growing Mycobacteria to 19 Antimicrobial Agents.BioMed research international · 2017Article
- Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples.Journal of clinical microbiology · 2016Article
- Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification.Journal of clinical microbiology · 2015Article
- Multi-probe real-time PCR identification of four common Candida species in blood culture broth.Mycopathologia · 2014Article
- Use of mycobacteriophage quantitative PCR on MGIT broths for a rapid tuberculosis antibiogram.Journal of clinical microbiology · 2014Article
- Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection.BMC infectious diseases · 2012Article
- Real-time PCR using mycobacteriophage DNA for rapid phenotypic drug susceptibility results for Mycobacterium tuberculosis.Journal of clinical microbiology · 2012Article
- Multiplex real-time PCR assay and melting curve analysis for identifying Mycobacterium tuberculosis complex and nontuberculous mycobacteria.Journal of clinical microbiology · 2012Article
- An appraisal of PCR-based technology in the detection of Mycobacterium tuberculosis.Molecular diagnosis & therapy · 2011Review
- Development and application of multiprobe real-time PCR method targeting the hsp65 gene for differentiation of Mycobacterium species from isolates and sputum specimens.Journal of clinical microbiology · 2010Article
- Combined real-time PCR and rpoB gene pyrosequencing for rapid identification of Mycobacterium tuberculosis and determination of rifampin resistance directly in clinical specimens.Journal of clinical microbiology · 2010Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium tuberculosis complex, M. avium, and M. intracellulare are the most common causes of systemic bacterial infection in AIDS patients. To identify these mycobacterial isolates in primary blood culture broths, we developed a multiple hybridization probe-based real-time PCR assay using the LightCycler system. The primers were designed to amplify a 320-bp fragment of Mycobacterium 16S rRNA genes. Reaction specificity was evaluated using PCR amplification curves along with specific melting temperatures of probes on DNA extracted from 13 Mycobacterium species. In this study, results showed 100% accuracy for the selected bacterial panel. Detection limits were 350, 600, and 650 colony-forming unit (CFU)/ml blood culture broths for M. tuberculosis complex, M. avium, and M. intracellulare, respectively (1 to 2 CFU/reaction). To evaluate clinical applicability, 341 acid-fast bacilli in blood culture broths were analyzed. In total, 327 (96%) were positively identified: 54.5% M. tuberculosis complex, 37.5% M. avium, and 3.8% M. intracellulare. Results can be available within 3 hours of receiving a broth sample, which makes this rapid and simple assay an attractive diagnostic tool for clinical use.
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