ArticleThe Journal of molecular diagnostics : JMD2009
Direct bacterial profiling by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for identification of pathogenic Neisseria.
Article in The Journal of molecular diagnostics : JMD, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- A Perspective on Artificial Intelligence for Molecular Pathologists.The Journal of molecular diagnostics : JMD · 2025Review
- Direct sub-atmospheric pressure ionization mass spectrometry: Evaporation/sublimation-driven ionization is amazing, fundamentally, and practically.Journal of mass spectrometry : JMS · 2024Review
- Monitoring of an Applied BeneficialMicroorganisms · 2023Article
- Neisseria montereyensis sp. nov., Isolated from Oropharynx of California Sea Lion (Zalophus californianus): Genomic, Phylogenetic, and Phenotypic Study.Current microbiology · 2023Article
- Whole Genome Analyses Accurately IdentifyInternational journal of molecular sciences · 2022Article
- Limitation ofJournal of clinical microbiology · 2022Article
- Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex.Molecules (Basel, Switzerland) · 2020Article
- Use of Andromas and Bruker MALDI-TOF MS in the identification of Neisseria.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Linear MALDI-ToF simultaneous spectrum deconvolution and baseline removal.BMC bioinformatics · 2018Article
- Identification of Neisseria gonorrhoeae by the Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System Is Improved by a Database Extension.Journal of clinical microbiology · 2016Article
- Evaluation of the Bruker MALDI Biotyper for Identification of Fastidious Gram-Negative Rods.Journal of clinical microbiology · 2016Article
- Identification of Microorganisms by High Resolution Tandem Mass Spectrometry with Accurate Statistical Significance.Journal of the American Society for Mass Spectrometry · 2016Article
- Meningitis and Bacteremia Due to Neisseria cinerea following a Percutaneous Rhizotomy of the Trigeminal Ganglion.Journal of clinical microbiology · 2016Article
- Proteomics of Neisseria gonorrhoeae: the treasure hunt for countermeasures against an old disease.Frontiers in microbiology · 2015Review
- The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota.Clinical microbiology reviews · 2015Article
- MALDI-TOF MS profiling approach: how much can we get from it?Frontiers in plant science · 2015Article
- MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.Frontiers in microbiology · 2015Review
- Identifying Neisseria species by use of the 50S ribosomal protein L6 (rplF) gene.Journal of clinical microbiology · 2014Article
- The construction and evaluation of reference spectra for the identification of human pathogenic microorganisms by MALDI-TOF MS.PloS one · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study investigates the suitability of direct bacterial profiling as a tool for the identification and subtyping of pathogenic Neisseria. The genus Neisseria includes two human pathogens, Neisseria meningitidis and Neisseria gonorrhoeae, as well as several nonpathogenic Neisseria species. Here, a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling protocol was optimized using a laboratory strain of E. coli DH5alpha to guarantee high quality and reproducible results. Subsequently, mass spectra for both laboratory and clinical strains of N. gonorrhoeae, N. meningitidis, and several nonpathogenic Neisseria species were collected. Significant interspecies differences but little intraspecies diversity were revealed by means of a visual inspection and bioinformatics examination using the MALDI BioTyper software. Cluster analysis successfully separated mass spectra collected from three groups that corresponded to N. gonorrhoeae, N. meningitidis, and nonpathogenic Neisseria isolates. Requiring only one bacterial colony for testing and using a fast and easy measuring protocol, this approach represents a powerful tool for the rapid identification of pathogenic Neisseria and can be adopted for other microorganisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.