ArticleTransgenic research2009
Caution! Analyze transcripts from conditional knockout alleles.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A new conditional mouse model for Cre-mediated restoration ofbioRxiv : the preprint server for biology · 2026Article
- Molecular characterization of Cdh12-SCON conditional knockout mice reveals unexpected splicing changes.Transgenic research · 2026Article
- Neuropathy target esterase (NTE/PNPLA6) and organophosphorus compound-induced delayed neurotoxicity (OPIDN).Advances in neurotoxicology · 2020Article
- Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice.FEBS letters · 2018Article
- Generation of a Tph2 Conditional Knockout Mouse Line for Time- and Tissue-Specific Depletion of Brain Serotonin.PloS one · 2015Article
- Severe hepatocellular disease in mice lacking one or both CaaX prenyltransferases.Journal of lipid research · 2012Article
- Measurement of protein farnesylation and geranylgeranylation in vitro, in cultured cells and in biopsies, and the effects of prenyl transferase inhibitors.Nature protocols · 2011Article
- Targeting protein prenylation for cancer therapy.Nature reviews. Cancer · 2011Article
- Article
- DHHC5 interacts with PDZ domain 3 of post-synaptic density-95 (PSD-95) protein and plays a role in learning and memory.The Journal of biological chemistry · 2010Article
- Genetic studies on the functional relevance of the protein prenyltransferases in skin keratinocytes.Human molecular genetics · 2010Article
- Targeting the protein prenyltransferases efficiently reduces tumor development in mice with K-RAS-induced lung cancer.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Are B-type lamins essential in all mammalian cells?Nucleus (Austin, Tex.)Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
A common strategy for conditional knockout alleles is to "flox" (flank with loxP sites) a 5' exon within the target gene. Typically, the floxed exon does not contain a unit number of codons so that the Cre-mediated recombination event yields a frameshift and a null allele. Documenting recombination within the genomic DNA is often regarded as sufficient proof of a frameshift, and the analysis of transcripts is neglected. We evaluated a previously reported conditional knockout allele for the beta-subunit of protein farnesyltransferase. The recombination event in that allele-the excision of exon 3-was predicted to yield a frameshift. However, following the excision of exon 3, exon 4 was skipped by the mRNA splicing machinery, and the predominant transcript from the mutant allele lacked exon 3 and exon 4 sequences. The "Deltaexon 3-4 transcript" does not contain a frameshift but rather is predicted to encode a protein with a short in-frame deletion. This represents a significant concern when studying an enzyme, since an enzyme with partial function could lead to erroneous conclusions. With thousands of new conditional knockout alleles under construction within mouse mutagenesis consortiums, the protein farnesyltransferase allele holds an important lesson-to characterize knockout alleles at both the DNA and RNA levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.