ArticleJournal of clinical microbiology2009
Utility of pyrosequencing in identifying bacteria directly from positive blood culture bottles.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 73 citations in OpenAlex.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- Molecular assays for the diagnosis of sepsis in neonates.The Cochrane database of systematic reviews · 2017Pooled it
- Isolation ofAfrican health sciences · 2019Article
- Campylobacter jejuni Fatal Sepsis in a Patient with Non-Hodgkin's Lymphoma: Case Report and Literature Review of a Difficult Diagnosis.International journal of molecular sciences · 2016Review
- Direct Screening of Blood by PCR and Pyrosequencing for a 16S rRNA Gene Target from Emergency Department and Intensive Care Unit Patients Being Evaluated for Bloodstream Infection.Journal of clinical microbiology · 2016Article
- Evaluation of real-time PCR and pyrosequencing for screening incubating blood culture bottles from adults with suspected bloodstream infection.Diagnostic microbiology and infectious disease · 2015Article
- New Molecular Techniques to Study the Skin Microbiota of Diabetic Foot Ulcers.Advances in wound care · 2015Review
- Developments for improved diagnosis of bacterial bloodstream infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Review
- Evaluation of MolYsis™ Complete5 DNA extraction method for detecting Staphylococcus aureus DNA from whole blood in a sepsis model using PCR/pyrosequencing.Journal of microbiological methods · 2014Article
- Early-onset neonatal sepsis.Clinical microbiology reviews · 2014Review
- Molecular-based screening for perinatal group B streptococcal infection: implications for prevention and therapy.Molecular diagnosis & therapy · 2013Review
- Molecular detection of culture-confirmed bacterial bloodstream infections with limited enrichment time.Journal of clinical microbiology · 2013Article
- Point of care investigations in pediatric care to improve health care in rural areas.Indian journal of pediatrics · 2013Article
- Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.PloS one · 2013Article
- DNAemia detection by multiplex PCR and biomarkers for infection in systemic inflammatory response syndrome patients.PloS one · 2012Article
- Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system.Journal of clinical microbiology · 2011Article
- Rapid identification of gram-negative bacteria with and without CTX-M extended-spectrum β-lactamase from positive blood culture bottles by PCR followed by microchip gel electrophoresis.Journal of clinical microbiology · 2011Article
- Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles.Journal of clinical microbiology · 2011Article
- Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2010Article
- Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Growth in liquid media is the gold standard for detecting microorganisms associated with bloodstream infections. The Gram stain provides the first clue as to the etiology of infection, with phenotypic identification completed 1 or 2 days later. Providing more detailed information than the Gram stain can impart, and in less time than subculturing, would allow the use of more directed empirical therapy and, thus, reduce the patient's exposure to unnecessary or ineffective antibiotics sooner. The study had two objectives, as follows: (i) to identify new targets to improve our ability to differentiate among certain enteric gram-negative rods or among certain Streptococcus species and (ii) to determine whether real-time PCR and pyrosequencing could as accurately identify organisms directly from positive blood culture bottles as culture-based methods. Two hundred and fifty-five consecutive positive blood culture bottles were included. The results showed a high level of agreement between the two approaches; of the 270 bacteria isolated from the 255 blood culture bottles, results for pyrosequencing and culture-based identifications were concordant for 264/270 (97.8%) bacteria with three failed sequences, and three sequences without match. Additionally, compared to the universal 16S rRNA gene target, the new 23S rRNA gene targets greatly improved our ability to differentiate among certain enteric gram-negative rods or among certain Streptococcus species. In conclusion, combining real-time PCR and pyrosequencing provided valuable information beyond that derived from the initial Gram stain and in less time than phenotypic culture-based identification. This strategy, if implemented, could result in a more directed empirical therapy in patients and would promote responsible antibiotic stewardship.
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