ArticleEpigenetics & chromatin2008
Single-epitope recognition imaging of native chromatin.
Article in Epigenetics & chromatin, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Cryo-EM structures of CENP-A nucleosomes isolated from human cells.Communications biology · 2026Article
- High-resolution analysis of human centromeric chromatin.Life science alliance · 2025Article
- Centromere structure and function: lessons from Drosophila.Genetics · 2023Article
- Molecular Complexes at Euchromatin, Heterochromatin and Centromeric Chromatin.International journal of molecular sciences · 2021Review
- Nano-Surveillance: Tracking Individual Molecules in a Sea of Chromatin.Journal of molecular biology · 2021Review
- Nucleosome-like, Single-stranded DNA (ssDNA)-Histone Octamer Complexes and the Implication for DNA Double Strand Break Repair.The Journal of biological chemistry · 2017Article
- Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points.The Journal of cell biology · 2017Article
- Application of catalyst-free click reactions in attaching affinity molecules to tips of atomic force microscopy for detection of protein biomarkers.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- The unconventional structure of centromeric nucleosomes.Chromosoma · 2012Review
- Article
- The CENP-A nucleosome: a dynamic structure and role at the centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- Tetrameric organization of vertebrate centromeric nucleosomes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Down the rabbit hole of centromere assembly and dynamics.Current opinion in cell biology · 2010Review
- Epigenetics & Chromatin celebrates its first anniversary.Epigenetics & chromatin · 2009Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundDirect visualization of chromatin has the potential to provide important insights into epigenetic processes. In particular, atomic force microscopy (AFM) can visualize single nucleosomes under physiological ionic conditions. However, AFM has mostly been applied to chromatin that has been reconstituted in vitro, and its potential as a tool for the dissection of native nucleosomes has not been explored. Recently we applied AFM to native Drosophila chromatin containing the centromere-specific histone 3 (CenH3), showing that it is greatly enriched in smaller particles. Taken together with biochemical analyses of CenH3 nucleosomes, we propose that centromeric nucleosomes are hemisomes, with one turn of DNA wrapped around a particle consisting of one molecule each of centromere-specific CenH3, H4, H2A and H2B.
resultsHere we apply a recognition mode of AFM imaging to directly identify CenH3 within histone core particles released from native centromeric chromatin. More than 90% of these particles were found to be tetrameric in height. The specificity of recognition was confirmed by blocking with a CenH3 peptide, and the strength of the interaction was quantified by force measurements. These results imply that the particles imaged by AFM are indeed mature CenH3-containing hemisomes.
conclusionEfficient and highly specific recognition of CenH3 in histone core particles isolated from native centromeric chromatin demonstrates that tetramers are the predominant form of centromeric nucleosomes in mature tetramers. Our findings provide proof of principle that this approach can yield insights into chromatin biology using direct and rapid detection of native nucleosomes in physiological salt concentrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.