ArticleActa biochimica et biophysica Sinica2008
Purification of melibiose-binding lectins from two cultivars of Chinese black soybeans.
Article in Acta biochimica et biophysica Sinica, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- Managing Anti-Nutritional Factors in Plant-Based Feeds: Implications for Herbivore Nutrition and Production.Metabolites · 2026Review
- Anti-Nutritional Factors of Plant Protein Feeds for Ruminants and Methods for Their Elimination.Animals : an open access journal from MDPI · 2025Review
- Lectins and lectibodies: potential promising antiviral agents.Cellular & molecular biology letters · 2022Review
- Regulation of Intestinal Inflammation by Soybean and Soy-Derived Compounds.Foods (Basel, Switzerland) · 2021Review
- Characterization of chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- GalNAc-Specific Soybean Lectin Inhibits HIV Infection of Macrophages through Induction of Antiviral Factors.Journal of virology · 2018Article
- The Influences of Soybean Agglutinin and Functional Oligosaccharides on the Intestinal Tract of Monogastric Animals.International journal of molecular sciences · 2018Review
- Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- A Glucosamine-Specific Lectin from Green Dragon No. 8 Beans (Phaseolus vulgaris) Induced Apoptosis on Nasopharyngeal Carcinoma Cells.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Antifungal and antiproliferative protein from Cicer arietinum: a bioactive compound against emerging pathogens.BioMed research international · 2014Article
- Article
- The development, characterization, and demonstration of a novel strategy for purification of recombinant proteins expressed in plants.Transgenic research · 2011Article
- High-yield expression of recombinant soybean agglutinin in plants using transient and stable systems.Transgenic research · 2011Article
- Lectins: production and practical applications.Applied microbiology and biotechnology · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A dimeric 50 kDa melibiose-binding lectin was isolated from the seeds of the cultivar of soybean (Glycine max), called the small glossy black soybean. The isolation procedure comprised ion exchange chromatography on Q Sepharose, SP Sepharose and Mono Q followed by gel filtration on Superdex 75. The lectin was adsorbed on all three ion exchangers, and it exhibited an N-terminal sequence identical to that of soybean lectin. Of all the sugars tested, melibiose most potently inhibited the hemagglutinating activity of the lectin, which was stable between pH 3-12 and 0-70 degrees C. The lectin evoked maximal mitogenic response at about the same molar concentration as Con A. However, the response was much weaker. The soybean lectin inhibited the activity of HIV-1 reverse transcriptase as well as the proliferation of breast cancer MCF7 cells and hepatoma HepG2 cells with an IC50 of 2.82 microM, 2.6 microM and 4.1 microM, respectively. There was no antifungal activity. Another lectin was isolated from a different cultivar of soybean called little black soybean. The lectin was essentially similar to small glossy black soybean lectin except for a larger subunit molecular mass (31 kDa), a more potent mitogenic activity and lower thermostability. The results indicate that different cultivars of soybean produce lectins that are not identical in every aspect.
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