ArticleApplied and environmental microbiology2009
A chromosomally located traHIJKCLMN operon encoding a putative type IV secretion system is involved in the virulence of Yersinia ruckeri.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- The Putative Virulence Plasmid pYR4 of the Fish PathogenBiology · 2024Article
- Pan-genome survey of the fish pathogen Yersinia ruckeri links accessory- and amplified genes to virulence.PloS one · 2023Article
- Stress response and virulence factors in bacterial pathogens relevant for Chilean aquaculture: current status and outlook of our knowledge.Biological research · 2022Review
- Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected WithFrontiers in immunology · 2021Article
- Phylogenetic Relatedness and Genome Structure ofFrontiers in microbiology · 2021Article
- Review
- The Infection Process ofFrontiers in cellular and infection microbiology · 2018Review
- Temperature-Dependent Gene Expression inFrontiers in microbiology · 2018Article
- Transcriptome and Proteome of Fish-PathogenicFrontiers in microbiology · 2018Article
- pYR4 From a Norwegian Isolate ofFrontiers in cellular and infection microbiology · 2018Article
- Comparative genome analysis reveals important genetic differences among serotype O1 and serotype O2 strains of Y. ruckeri and provides insights into host adaptation and virulence.MicrobiologyOpen · 2017Article
- A Synonymous Mutation Upstream of the Gene Encoding a Weak-Link Enzyme Causes an Ultrasensitive Response in Growth Rate.Journal of bacteriology · 2016Article
- Genome Sequence of the Fish Pathogen Yersinia ruckeri SC09 Provides Insights into Niche Adaptation and Pathogenic Mechanism.International journal of molecular sciences · 2016Article
- Complete Genome Sequence of the Fish Pathogen Yersinia ruckeri Strain SC09, Isolated from Diseased Ictalurus punctatus in China.Genome announcements · 2015Article
- Temperature-dependent expression of virulence genes in fish-pathogenic bacteria.Frontiers in microbiology · 2015Review
- The heat sensitive factor (HSF) of Yersinia ruckeri is produced by an alkyl sulphatase involved in sodium dodecyl sulphate (SDS) degradation but not in virulence.BMC microbiology · 2014Article
- A novel cdsAB operon is involved in the uptake of L-cysteine and participates in the pathogenesis of Yersinia ruckeri.Journal of bacteriology · 2011Article
- The yctCBA operon of Yersinia ruckeri, involved in in vivo citrate uptake, is not required for virulence.Applied and environmental microbiology · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleotide sequence analysis of the region surrounding the pIVET8 insertion site in Yersinia ruckeri 150RiviXII, previously selected by in vivo expression technology (IVET), revealed the presence of eight genes (traHIJKCLMN [hereafter referred to collectively as the tra operon or tra cluster]), which are similar both in sequence and organization to the tra operon cluster found in the virulence-related plasmid pADAP from Serratia entomophila. Interestingly, the tra cluster of Y. ruckeri is chromosomally encoded, and no similar tra cluster has been identified yet in the genomic analysis of human pathogenic yersiniae. A traI insertional mutant was obtained by homologous recombination. Coinfection experiments with the mutant and the parental strain, as well as 50% lethal dose determinations, indicate that this operon is involved in the virulence of this bacterium. All of these results suggest the implication of the tra cluster in a virulence-related type IV secretion/transfer system. Reverse transcriptase PCR studies showed that this cluster is transcribed as an operon from a putative promoter located upstream of traH and that the mutation of traI had a polar effect. A traI::lacZY transcriptional fusion displayed higher expression levels at 18 degrees C, the temperature of occurrence of the disease, and under nutrient-limiting conditions. PCR detection analysis indicated that the tra cluster is present in 15 Y. ruckeri strains from different origins and with different plasmid profiles. The results obtained in the present study support the conclusion, already suggested by different authors, that Y. ruckeri is a very homogeneous species that is quite different from the other members of the genus Yersinia.
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