ArticleApplied and environmental microbiology2009
Linking sequence to function in soil bacteria: sequence-directed isolation of novel bacteria contributing to soilborne plant disease suppression.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
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- Metagenomic analysis of soybean endosphere microbiome to reveal signatures of microbes for health and disease.Journal, genetic engineering & biotechnology · 2023Article
- Genome analysis provides insights into the biocontrol ability of Mitsuaria sp. strain TWR114.Archives of microbiology · 2021Article
- Wheat Genotype-Specific Recruitment of Rhizosphere Bacterial Microbiota Under Controlled Environments.Frontiers in plant science · 2021Article
- Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease.Frontiers in microbiology · 2021Article
- Microbial Origin of Aquaponic Water Suppressiveness againstMicroorganisms · 2020Article
- Diversity and Structure of the Endophytic Bacterial Communities Associated With Three Terrestrial Orchid Species as Revealed by 16S rRNA Gene Metabarcoding.Frontiers in microbiology · 2020Article
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- Plants Assemble Species Specific Bacterial Communities from Common Core Taxa in Three Arcto-Alpine Climate Zones.Frontiers in microbiology · 2017Article
- The Bacterial Community Structure and Dynamics of Carbon and Nitrogen when Maize (Zea mays L.) and Its Neutral Detergent Fibre Were Added to Soil from Zimbabwe with Contrasting Management Practices.Microbial ecology · 2017Article
- What's Inside That Seed We Brew? A New Approach To Mining the Coffee Microbiome.Applied and environmental microbiology · 2015Review
- Soil Conditions That Can Alter Natural Suppression of Escherichia coli O157:H7 in Ohio Specialty Crop Soils.Applied and environmental microbiology · 2015Article
- Association of shifting populations in the root zone microbiome of millet with enhanced crop productivity in the Sahel region (Africa).Applied and environmental microbiology · 2015Article
- Quantitative divergence of the bacterial root microbiota in Arabidopsis thaliana relatives.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing.PloS one · 2014Article
- Role of bacterial communities in the natural suppression of Rhizoctonia solani bare patch disease of wheat (Triticum aestivum L.).Applied and environmental microbiology · 2013Article
- Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development.The plant pathology journal · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial community profiling of samples differing in a specific ecological function, i.e., soilborne plant disease suppression, can be used to mark, recover, and ultimately identify the bacteria responsible for that specific function. Previously, several terminal restriction fragments (TRF) of 16S rRNA genes were statistically associated with damping-off disease suppression. This work presents the development of sequence-based TRF length polymorphism (T-RFLP)-derived molecular markers to direct the identification and isolation of novel bacteria involved in damping-off pathogen suppression. Multiple sequences matching TRF M139 and M141 were cloned and displayed identity to multiple database entries in the genera incertae sedis of the Burkholderiales. Sequences matching TRF M148, in contrast, displayed greater sequence diversity. A sequence-directed culturing strategy was developed using M139- and M141-derived markers and media reported to be selective for the genera identified within this group. Using this approach, we isolated and identified novel Mitsuaria and Burkholderia species with high levels of sequence similarity to the targeted M139 and M141 TRF, respectively. As predicted, these Mitsuaria and Burkholderia isolates displayed the targeted function by reducing fungal and oomycete plant pathogen growth in vitro and reducing disease severity in infected tomato and soybean seedlings. This work represents the first successful example of the use of T-RFLP-derived markers to direct the isolation of microbes with pathogen-suppressing activities, and it establishes the power of low-cost molecular screening to identify and direct the recovery of functionally important microbes, such as these novel biocontrol strains.
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