ArticleThe Journal of biological chemistry2009
Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Functional mutations in the thyroid-stimulating hormone receptor in natural stickleback populations at sites identical to human disease-causing mutations.BMC ecology and evolution · 2025Article
- PAQR4 regulates adipocyte function and systemic metabolic health by mediating ceramide levels.Nature metabolism · 2024Article
- Effect of Empagliflozin on Sphingolipid Catabolism in Diabetic and Hypertensive Rats.International journal of molecular sciences · 2022Article
- Miocene Diversification and High-Altitude Adaptation ofInsects · 2020Article
- A neutral ceramidase, NlnCDase, is involved in the stress responses of brown planthopper, Nilaparvata lugens (Stål).Scientific reports · 2018Article
- Article
- Molecular mechanism for sphingosine-induced Pseudomonas ceramidase expression through the transcriptional regulator SphR.Scientific reports · 2016Article
- Structural Basis for Ceramide Recognition and Hydrolysis by Human Neutral Ceramidase.Structure (London, England : 1993) · 2015Article
- Catalytic Mechanism of Eukaryotic Neutral Ceramidase.Structure (London, England : 1993) · 2015Article
- Sphingosine-1-phosphate metabolism: A structural perspective.Critical reviews in biochemistry and molecular biology · 2015Review
- Pseudomonas-derived ceramidase induces production of inflammatory mediators from human keratinocytes via sphingosine-1-phosphate.PloS one · 2014Article
- A review of ceramide analogs as potential anticancer agents.Future medicinal chemistry · 2013Review
- Purification and biochemical characterization of membrane-bound neutral ceramidase from camel brain (Camelus dromedarius).International journal of biochemistry and molecular biology · 2013Article
- PLP-dependent enzymes as entry and exit gates of sphingolipid metabolism.Protein science : a publication of the Protein Society · 2011Review
- CREST--a large and diverse superfamily of putative transmembrane hydrolases.Biology direct · 2011Article
- Molecular cloning and characterization of neutral ceramidase homologue from the red flour beetle, Tribolium castaneum.Biochimie · 2011Article
- Drug targeting of sphingolipid metabolism: sphingomyelinases and ceramidases.British journal of pharmacology · 2011Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ceramidase (CDase; EC 3.5.1.23) hydrolyzes ceramide to generate sphingosine and fatty acid. The enzyme plays a regulatory role in a variety of physiological events in eukaryotes and also functions as an exotoxin in particular bacteria. The crystal structures of neutral CDase from Pseudomonas aeruginosa (PaCD) in the C2-ceramide-bound and -unbound forms were determined at 2.2 and 1.4 A resolutions, respectively. PaCD consists of two domains, and the Zn(2+)- and Mg(2+)/Ca(2+)-binding sites are found within the center of the N-terminal domain and the interface between the domains, respectively. The structural comparison between the C2-ceramide-bound and unbound forms revealed an open-closed conformational change occurring to loop I upon binding of C2-ceramide. In the closed state, this loop sits above the Zn(2+) coordination site and over the opening to the substrate binding site. Mutational analyses of residues surrounding the Zn(2+) of PaCD and rat neutral CDase revealed that the cleavage or creation of the N-acyl linkage of ceramide follows a similar mechanism as observed for the Zn(2+)-dependent carboxypeptidases. The results provide an understanding of the molecular mechanism of hydrolysis and synthesis of ceramide by the enzyme. Furthermore, insights into the actions of PaCD and eukaryotic neutral CDases as an exotoxin and mediators of sphingolipid signaling are also revealed, respectively.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.