ArticleGenetics2009
Ribosome association and stability of the nascent polypeptide-associated complex is dependent upon its own ubiquitination.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- The CCR4-NOT complex: a multifaceted sensor of molecular signals instructing eukaryotic mRNA translation and stability.Nucleic acids research · 2025Review
- Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases.Biochemistry and biophysics reports · 2024Article
- Not4-dependent targeting of MMF1 mRNA to mitochondria limits its expression via ribosome pausing, Egd1 ubiquitination, Caf130, no-go-decay and autophagy.Nucleic acids research · 2023Article
- Review
- Molecular Characterization of the Oncogene BTF3 and Its Targets in Colorectal Cancer.Frontiers in cell and developmental biology · 2020Article
- A DREPP protein interacted with PeaT1 from Alternaria tenuissima and is involved in elicitor-induced disease resistance in Nicotiana plants.Journal of plant research · 2018Article
- Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA.Nucleic acids research · 2017Article
- Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis.PLoS genetics · 2015Article
- OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria.Nature communications · 2014Article
- Differential expression of microRNAs in dormant bud of tea [Camellia sinensis (L.) O. Kuntze].Plant cell reports · 2014Article
- Protecting the proteome: Eukaryotic cotranslational quality control pathways.The Journal of cell biology · 2014Review
- Novel roles of the multi-functional CCR4-NOT complex in post-transcriptional regulation.Frontiers in genetics · 2014Review
- The Not4 E3 ligase and CCR4 deadenylase play distinct roles in protein quality control.PloS one · 2014Article
- The role of the E3 ligase Not4 in cotranslational quality control.Frontiers in genetics · 2014Review
- The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control.ISRN molecular biology · 2013Review
- The control of elongation by the yeast Ccr4-not complex.Biochimica et biophysica acta · 2013Review
- The ubiquitin-proteasome system of Saccharomyces cerevisiae.Genetics · 2012Review
- The essential Ubc4/Ubc5 function in yeast is HECT E3-dependent, and RING E3-dependent pathways require only monoubiquitin transfer by Ubc4.The Journal of biological chemistry · 2011Article
- Not4 E3 ligase contributes to proteasome assembly and functional integrity in part through Ecm29.Molecular and cellular biology · 2011Article
- Cytoplasmic deadenylation: regulation of mRNA fate.Biochemical Society transactions · 2010Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work we addressed the role of ubiquitination in the function of the nascent polypeptide-associated complex (NAC), named EGD in the yeast Saccharomyces cerevisiae. To this end, we first identified the lysines residues required for ubiquitination of EGD/NAC. While simultaneous mutation of many lysines in the alpha-subunit of NAC (Egd2p) was required to abolish its ubiquitination, for the beta-subunit of NAC (Egd1p), mutation of K29 and K30 was sufficient. We determined that the ubiquitination of the two EGD subunits was coordinated, occurring during growth first on Egd1p and then on Egd2p. Egd2p was ubiquitinated earlier during growth if Egd1p could not be ubiquitinated. The use of mutants revealed the importance of EGD ubiqutination for its ribosome association and stability. Finally, our study demonstrated an interaction of EGD/NAC with the proteasome and revealed the importance of the Not4p E3 ligase, responsible for EGD/NAC ubiquitination, in this association.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.