ArticleFEMS immunology and medical microbiology2009
Substitution of methionine 63 or 83 in S100A9 and cysteine 42 in S100A8 abrogate the antifungal activities of S100A8/A9: potential role for oxidative regulation.
Article in FEMS immunology and medical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
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- The Role of Zinc in Gliotoxin Biosynthesis ofInternational journal of molecular sciences · 2019Article
- The relationship between DJ-1 and S100A8 in human primary alveolar type II cells in emphysema.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Secretion of the Phosphorylated Form of S100A9 from Neutrophils Is Essential for the Proinflammatory Functions of Extracellular S100A8/A9.Frontiers in immunology · 2018Article
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- S100 Proteins As an Important Regulator of Macrophage Inflammation.Frontiers in immunology · 2017Review
- Identification of proteins from 4200-year-old skin and muscle tissue biopsies from ancient Egyptian mummies of the first intermediate period shows evidence of acute inflammation and severe immune response.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans.Iranian journal of pharmaceutical research : IJPR · 2014Article
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- S100A8 and S100A9: DAMPs at the crossroads between innate immunity, traditional risk factors, and cardiovascular disease.Mediators of inflammation · 2013Review
- Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry.Molecular & cellular proteomics : MCP · 2012Article
- Innate antifungal immunity: the key role of phagocytes.Annual review of immunology · 2011Review
- S-glutathionylation regulates inflammatory activities of S100A9.The Journal of biological chemistry · 2010Article
- ANTI-INFECTIVE PROTECTIVE PROPERTIES OF S100 CALGRANULINS.Anti-inflammatory & anti-allergy agents in medicinal chemistry · 2009Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
S100A8 and S100A9 and their heterocomplex calprotectin (S100A8/A9) are abundant cytosolic constituents in human neutrophils previously shown to possess antifungal activity. This study was designed to investigate mechanisms involved in the modulation of the antifungal properties of S100A8/A9. S100A8, S100A9 and site-directed mutants of both proteins were tested for their antifungal effect against Candida albicans in microplate dilution assays. Whereas S100A8 alone did not inhibit fungal growth, S100A9 by itself had a moderate antifungal effect. Combining both proteins had the strongest effect. Supporting a potential role for oxidation in S100A8/A9, substitution of methionine 63 or 83 of S100A9 resulted in the loss of antifungal activity. Additionally, the substitution to alanine of cysteine 42 of S100A8 also caused a loss of S100A8's ability to enhance S100A9's antifungal effect. Overall, our data indicate that both S100A8 and S100A9 are required for their fully active antifungal effect and that oxidation regulates S100A8/A9 antifungal activity through mechanisms that remain to be elucidated and evaluated. Finally, together with our previous work describing the oxidation-sensitive anti-inflammatory effects of S100A8/A9, we propose that S100A8/A9 exerts an anti-inflammatory activity in healthy state and that conditions associated with oxidative stress activate the antifungal activity of S100A8/A9.
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Registered trials
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