ArticlePlanta2009
Reciprocal chromosome translocation associated with TDNA-insertion mutation in Arabidopsis: genetic and cytological analyses of consequences for gametophyte development and for construction of doubly mutant lines.
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- A complex reciprocal translocation is linked to reduced gamete viability in a loose-bunch grapevine somatic variant.BMC plant biology · 2026Article
- Structural defects in male meiotic cell division lead to reproductive genome instability and disturbed microsporogenesis in banana (Frontiers in plant science · 2026Article
- T-DNAreader: fast and precise identification of T-DNA insertion sites in plant genomes using RNA sequencing data.Genome biology · 2025Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Reproductive defects in the abscission mutant ida-2 are caused by T-DNA-induced genomic rearrangements.Plant physiology · 2023Article
- Transformation and regeneration of DNA polymerase Θ mutant rice plants.Plant direct · 2023Article
- Characterization of T-Circles and Their Formation Reveal Similarities toFrontiers in plant science · 2022Article
- Exploring Genomic Variations in Nematode-Resistant Mutant Rice Lines.Frontiers in plant science · 2022Article
- Plant DNA Repair andInternational journal of molecular sciences · 2021Review
- Large scale genomic rearrangements in selected Arabidopsis thaliana T-DNA lines are caused by T-DNA insertion mutagenesis.BMC genomics · 2021Article
- Identification and characterization of a female gametophyte defect inPlant signaling & behavior · 2020Article
- Gametophytic Abortion in Heterozygotes but Not in Homozygotes: Implied Chromosome Rearrangement during T-DNA Insertion at theMolecules and cells · 2020Article
- Comprehensive characterization of T-DNA integration induced chromosomal rearrangement in a birch T-DNA mutant.BMC genomics · 2019Article
- Article
- Clearance of maternal barriers by paternal miR159 to initiate endosperm nuclear division in Arabidopsis.Nature communications · 2018Article
- Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere.Plant cell reports · 2017Article
- Genome-Wide Assessment of Efficiency and Specificity in CRISPR/Cas9 Mediated Multiple Site Targeting in Arabidopsis.PloS one · 2016Article
- The Translesion Polymerase ζ Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis.Plant physiology · 2015Article
- A novel T-DNA integration in rice involving two interchromosomal translocations.Plant cell reports · 2014Article
- The classical arabinogalactan protein AGP18 mediates megaspore selection in Arabidopsis.The Plant cell · 2013Article
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7 authors.
Funding
Abstract
Chromosomal rearrangements may complicate construction of Arabidopsis with multiple TDNA-insertion mutations. Here, crossing two lines homozygous for insertions in AtREV3 and AtPOLH (chromosomes I and V, respectively) and selfing F1 plants yielded non-Mendelian F2 genotype distributions: frequencies of +/++/+ and 1/1 2/2 progeny were only 0.42 and 0.25%. However, the normal development and fertility of double mutants showed AtPOLH-1 and AtREV3-2 gametes and 1/1 2/2 embryos to be fully viable. F2 distributions could be quantitatively predicted by assuming that F1 selfing produced inviable (1,2) and (+,+) gametophytes 86% of the time. Some defect intrinsic to the F1 selfing process itself thus appeared responsible. In selfing AtREV3 (+/2 ) single mutants, imaging of ovules and pollen showed arrest or abortion, respectively, of half of gametophytes; however, gametogenesis was normal in AtREV3 ( 2/2 ) homozygotes. These findings, taken together, suggested that T-DNA insertion at AtREV3 on chromosome I had caused a reciprocal I-V translocation. Spreads of meiosis I chromosomes in selfing AtREV3 (+/2 ) heterozygotes revealed the predicted cruciform four-chromosome structures, which fluorescence in situ hybridization showed to invariably include both translocated and normal chromosomes I and V. Sequencing of the two junctions of T-DNA with AtREV3 DNA and the two with gene At5g59920 suggested translocation via homologous recombination between independent inverted-repeat T-DNA insertions. Thus, when crosses between TDNA-insertion mutants yield anomalous progeny distributions, TDNA-linked translocations should be considered.
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