ArticleProceedings of the National Academy of Sciences of the United States of America1991
Embryonic stem cells lacking a functional inhibitory G-protein subunit (alpha i2) produced by gene targeting of both alleles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 89 citations in OpenAlex.
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- Efficient KRT14 targeting and functional characterization of transplanted human keratinocytes for the treatment of epidermolysis bullosa simplex.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- alpha-Catenin as a tension transducer that induces adherens junction development.Nature cell biology · 2010Article
- Evidence for a role of vertebrate Disp1 in long-range Shh signaling.Development (Cambridge, England) · 2010Article
- Pooling and PCR as a method to combat low frequency gene targeting in mouse embryonic stem cells.Cytotechnology · 2006Article
- Cancer predisposition and hematopoietic failure in Rad50(S/S) mice.Genes & development · 2002Article
- Dual roles of Cripto as a ligand and coreceptor in the nodal signaling pathway.Molecular and cellular biology · 2002Article
- Distinct retinoid metabolic functions for alcohol dehydrogenase genes Adh1 and Adh4 in protection against vitamin A toxicity or deficiency revealed in double null mutant mice.The Journal of biological chemistry · 2002Article
- Apoptotic protease activating factor 1 (Apaf-1)-independent cell death suppression by Bcl-2.The Journal of experimental medicine · 2000Article
- Involvement of Gialpha2 in sodium butyrate-induced erythroblastic differentiation of K562 cells.The Biochemical journal · 2000Article
- Simultaneous Cre catalyzed recombination of two alleles to restore neomycin sensitivity and facilitate homozygous mutations.Nucleic acids research · 1999Article
- Phosphorylation of arylsulphatase A occurs through multiple interactions with the UDP-N-acetylglucosamine-1-phosphotransferase proximal and distal to its retrieval site by the KDEL receptor.The Biochemical journal · 1999Article
- Gene targeting of retinoid receptors.Molecular biotechnology · 1999Article
- Enhanced bradykinin-stimulated phospholipase C activity in murine embryonic stem cells lacking the G-protein alphaq-subunit.The Biochemical journal · 1997Article
- Targeted inactivation of alphai2 or alphai3 disrupts activation of the cardiac muscarinic K+ channel, IK+Ach, in intact cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Targeted disruption of the mouse topoisomerase I gene by camptothecin selection.Molecular and cellular biology · 1996Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The alpha i2 subunit of the inhibitory heterotrimeric guanine nucleotide-binding proteins is highly conserved in mammals and is expressed in all cell types, but its exact function is not yet defined. We have investigated the role of this protein by producing embryonic stem (ES) cells lacking a functional alpha i2 gene. These alpha i2-null cell lines regulate adenylyl cyclase and grow and differentiate in vitro the same as wild-type ES cells. Homologous recombination was used to sequentially inactivate both copies of the alpha i2 gene. The first allele was inactivated by insertion of a neomycin-resistance gene. We modified the hygromycin B-resistance gene for improved expression in ES cells and used this gene to inactivate the remaining normal allele. The techniques used should be generally applicable to other genes whether or not they are expressed in ES cells.
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