ArticlePloS one2008
Effects of intracellular calcium and actin cytoskeleton on TCR mobility measured by fluorescence recovery.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- Combined TIRF and 3D Super-Resolution Microscopy for Nanoscopic Characterization of Adhesion Molecules on Microvilli.Analytical chemistry · 2026Article
- TCR-pMHC recognition mediates target phagocytosis by T cells.Mechanobiology in medicine · 2026Article
- Transient receptor potential vanilloid 4 modulates substrate stiffness mechanosensing and transcellular pore formation in human Schlemm's canal cells.Acta biomaterialia · 2025Article
- TRPV4 modulates substrate stiffness mechanosensing and transcellular pore formation in human Schlemm's canal cells.bioRxiv : the preprint server for biology · 2025Article
- Disruption of P2Y2 Signaling Promotes Breast Tumor Cell Dissemination by Reducing ATP-Dependent Calcium Elevation and Actin Localization to Cell Junctions.International journal of molecular sciences · 2025Article
- An Immune-Independent Mode of Action of Tacrolimus in Promoting Human Extravillous Trophoblast Migration Involves Intracellular Calcium Release and F-Actin Cytoskeletal Reorganization.International journal of molecular sciences · 2024Article
- Hepatitis B virus X protein enhances liver cancer cell migration by regulating calmodulin-associated actin polymerization.BMB reports · 2021Article
- Stationed or Relocating: The Seesawing EMT/MET Determinants from Embryonic Development to Cancer Metastasis.Biomedicines · 2021Review
- Extracellular Calcium Ion Concentration Regulates Chondrocyte Elastic Modulus and Adhesion Behavior.International journal of molecular sciences · 2021Article
- Microclusters as T Cell Signaling Hubs: Structure, Kinetics, and Regulation.Frontiers in cell and developmental biology · 2020Review
- Membrane Organization and Physical Regulation of Lymphocyte Antigen Receptors: A Biophysicist's Perspective.The Journal of membrane biology · 2019Review
- The Influence of Molecular Reach and Diffusivity on the Efficacy of Membrane-Confined Reactions.Biophysical journal · 2019Article
- TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps.Nature communications · 2019Article
- TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells.Nature immunology · 2018Article
- Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation.Science advances · 2017Article
- Cancer cells become less deformable and more invasive with activation of β-adrenergic signaling.Journal of cell science · 2016Article
- Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Calcium influx through CRAC channels controls actin organization and dynamics at the immune synapse.eLife · 2016Article
- Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Distinct Roles of Cytoskeletal Components in Immunological Synapse Formation and Directed Secretion.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe activation of T lymphocytes by specific antigen is accompanied by the formation of a specialized signaling region termed the immunological synapse, characterized by the clustering and segregation of surface molecules and, in particular, by T cell receptor (TCR) clustering. METHODOLOGY/PRINCIPAL
findingsTo better understand TCR motion during cellular activation, we used confocal microscopy and photo-bleaching recovery techniques to investigate the lateral mobility of TCR on the surface of human T lymphocytes under various pharmacological treatments. Using drugs that cause an increase in intracellular calcium, we observed a decrease in TCR mobility that was dependent on a functional actin cytoskeleton. In parallel experiments measurement of filamentous actin by FACS analysis showed that raising intracellular calcium also causes increased polymerization of the actin cytoskeleton. These in vitro results were analyzed using a mathematical model that revealed effective binding parameters between TCR and the actin cytoskeleton. CONCLUSION/SIGNIFICANCE: We propose, based on our results, that increase in intracellular calcium levels leads to actin polymerization and increases TCR/cytoskeleton interactions that reduce the overall mobility of the TCR. In a physiological setting, this may contribute to TCR re-positioning at the immunological synapse.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.