ArticleActa pharmacologica Sinica2009
Transformation and action of extracellular NAD+ in perfused rat and mouse livers.
Article in Acta pharmacologica Sinica, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 8 citations in OpenAlex.
- Sexual Dimorphism of Ethanol-Induced Mitochondrial Dynamics in Purkinje Cells.International journal of molecular sciences · 2024Article
- A sense of proximity: Cell packing modulates oxygen consumption.APL bioengineering · 2023Article
- Insulin degludec and glutamine dipeptide modify glucose homeostasis and liver metabolism in diabetic mice undergoing insulin-induced hypoglycemia.Journal of applied biomedicine · 2021Article
- Nicotinamide mononucleotide alters mitochondrial dynamics by SIRT3-dependent mechanism in male mice.Journal of neuroscience research · 2019Article
- Co-regulation of primary mouse hepatocyte viability and function by oxygen and matrix.Biotechnology and bioengineering · 2014Article
- Perfused multiwell plate for 3D liver tissue engineering.Lab on a chip · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
aimTransformation and possible metabolic effects of extracellular NAD+ were investigated in the livers of mice (Mus musculus; Swiss strain) and rats (Rattus novergicus; Holtzman and Wistar strains).
methodsThe livers were perfused in an open system using oxygen-saturated Krebs/Henseleit-bicarbonate buffer (pH 7.4) as the perfusion fluid. The transformation of NAD+ was monitored using high-performance liquid chromatography.
resultsIn the mouse liver, the single-pass metabolism of 100 micromol/L NAD+ was almost complete; ADP-ribose and nicotinamide were the main products in the outflowing perfusate. In the livers of both Holtzman and Wistar rats, the main transformation products were ADP-ribose, uric acid and nicotinamide; significant amounts of inosine and AMP were also identified. On a weight basis, the transformation of NAD+ was more efficient in the mouse liver. In the rat liver, 100 micromol/L NAD+ transiently inhibited gluconeogenesis and oxygen uptake. Inhibition was followed by a transient stimulation. Inhibition was more pronounced in the Wistar strain and stimulation was more pronounced in the Holtzman strain. In the mouse liver, no clear effects on gluconeogenesis and oxygen uptake were found even at 500 micromol/L NAD+.
conclusionIt can be concluded that the functions of extracellular NAD+ are species-dependent and that observations in one species are strictly valid for that species. Interspecies extrapolations should thus be made very carefully. Actually, even variants of the same species can demonstrate considerably different responses.
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