ArticleCell and tissue research1991
Myofibrillar and cytoskeletal assembly in neonatal rat cardiac myocytes cultured on laminin and collagen.
Article in Cell and tissue research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 72 citations in OpenAlex.
- Stem Cell Differentiation into Cardiomyocytes: Current Methods and Emerging Approaches.Stem cell reviews and reports · 2022Review
- DNA Tension Probes Show that Cardiomyocyte Maturation Is Sensitive to the Piconewton Traction Forces Transmitted by Integrins.ACS nano · 2022Article
- Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes.Theranostics · 2018Review
- Article
- Heart regeneration with engineered myocardial tissue.Annual review of biomedical engineering · 2014Review
- Cardiogel: a nano-matrix scaffold with potential application in cardiac regeneration using mesenchymal stem cells.PloS one · 2014Article
- Human embryonic stem cell-derived cardiomyocytes migrate in response to gradients of fibronectin and Wnt5a.Stem cells and development · 2013Article
- Regeneration in heart disease-Is ECM the key?Life sciences · 2012Review
- Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function.Pflugers Archiv : European journal of physiology · 2011Review
- Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.PloS one · 2010Article
- Intramyocardial fibroblast myocyte communication.Circulation research · 2010Review
- Sarcomere alignment is regulated by myocyte shape.Cell motility and the cytoskeleton · 2008Article
- Mechanotransduction in skeletal muscle.Frontiers in bioscience : a journal and virtual library · 2007Review
- Dynamics of Z-band based proteins in developing skeletal muscle cells.Cell motility and the cytoskeleton · 2005Article
- Cardiogel: a biosynthetic extracellular matrix for cardiomyocyte culture.In vitro cellular & developmental biology. Animal · 1996Article
- Article
- Immunofluorescent studies on Z-line-associated protein in cultured cardiomyocytes from neonatal hamsters.Cell and tissue research · 1993Article
- Costameres are sites of force transmission to the substratum in adult rat cardiomyocytes.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neonatal rat cardiomyocytes were cultured on extracellular matrix components laminin and collagens I + III to examine effects of extracellular matrix on the assembly of cytoskeletal proteins during myofibrillogenesis. Myofibril assembly was visualized by immunofluorescence of marker proteins for myofibrils (f-actin for I bands and alpha-actinin for Z bands), focal adhesions (vinculin), and transmembrane extracellular matrix receptors (beta 1 integrin) as cells spread for various times in culture. By 4 h in culture, f-actin appeared organized into nonstriated stress-fiber-like structures while alpha-actinin, vinculin and beta 1 integrin were localized in small streaks and beads. Subsequently, striated patterns were observed sequentially in the intracellular cytoskeletal components alpha-actinin, vinculin, f-actin, and then in the transmembrane beta 1 integrin receptor. These data support an earlier model for sarcomerogenesis in which stress-fiber-like structures serve as initial scaffolds upon which alpha-actinin and then vinculin-containing costameres are assembled. This sequential and temporal assembly was the same on both laminin and collagens I + III. A quantitative difference, however, was apparent on the 2 matrices. There was an increased appearance on collagens I + III of rosettes (also called podosomes or cortical actin-containing bodies in other cells) which consisted of an f-actin core surrounded by alpha-actinin, vinculin and beta 1 integrin rims. The increased incidence of rosettes in neonatal myocytes on collagens I + III suggests that these cytoskeletal complexes are involved in recognition and interaction with extracellular matrix components.
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